生物素Anti-sRANKL抗体(ab83145)
Key features and details
- Biotin Rabbit polyclonal to sRANKL
- Suitable for: Sandwich ELISA, WB
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-sRANKL抗体
参阅全部 sRANKL 一抗 -
描述
生物素兔多克隆抗体to sRANKL -
宿主
Rabbit -
偶联物
Biotin -
经测试应用
适用于: Sandwich ELISA, WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Highly pure (>98%) recombinant human sRANKL.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Centrifuge vial prior to opening. Reconstitute in 50 µl sterile PBS containing 0.1% BSA to give a concentration of 1.0 mg/ml. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab83145 is sterile filtered. ab83145 was purified by affinity chromatography and then biotinylated. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab83145于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human sRANKL. |
|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa.
The detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions. |
| 说明 |
|---|
|
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human sRANKL. |
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions. |
靶标
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功能
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
组织特异性
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
疾病相关
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
细胞定位
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
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别名
- CD254 antibody
- hRANKL2 antibody
- ODF antibody
see all
图片
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Western blot - Biotin Anti-sRANKL antibody (ab83145)
To detect human sRANKL by Western Blot analysis ab83145 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant sRANKL protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant sRANKL protein.
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Sandwich ELISA - Biotin Anti-sRANKL antibody (ab83145)To detect human sRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83145 is required. This biotinylated polyclonal antibody, in conjunction with an anti-RANKL (ab9957) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human sRANKL.
数据表及文件
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Datasheet download
文献 (0)
ab83145 尚未被引用在任何文献中。
