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AB271279

生物素Anti-SDF1 beta抗体

Biotin Anti-SDF1 beta antibody

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(1 Publication)

Anti-SDF1 beta antibody conjugated to Biotin is a goat polyclonal antibody suitable for Western blot, sELISA.

查看别名

SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF

2 Images
Sandwich ELISA - Biotin Anti-SDF1 beta antibody (AB271279)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-SDF1 beta antibody (AB271279)

Human SDF1 beta was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271279.

This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant SDF1 beta protein.

Western blot - Biotin Anti-SDF1 beta antibody (AB271279)
  • WB

Supplier Data

Western blot - Biotin Anti-SDF1 beta antibody (AB271279)

To detect Human SDF-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml.

When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Lane 1 : Marker

Lanes 2-12 : 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human SDF1 beta, respectively.

Non-reducing conditions.

All lanes:

Western blot - Biotin Anti-SDF1 beta antibody (ab271279)

Predicted band size: 11 kDa

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关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

偶联物

Biotin

激发波长/发射波长
不含载体蛋白

No

应用

WB, sELISA

applications

免疫原

Recombinant Fragment Protein within Human CXCL12 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P48061

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搭配使用产品

Assay Kits

AB100637

Human SDF1 ELISA Kit

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)

  • 5
  • 2
Proteins & Peptides

AB259416

Recombinant human SDF1 protein (Active)

Premium Bioactive

Functional Studies - Recombinant human SDF1 protein (Active) (AB259416)

  • 0
  • 0
Primary Antibodies

AB124824

Anti-CXCR4 antibody [UMB2]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR4 antibody [UMB2] (AB124824)

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  • 17
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p>When used in conjunction with compatible secondary reagents, the detection limit for recombinant human SDF1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Lyophilized
复溶
Reconstitute in PBS, 0.1% BSA
纯化工艺
Affinity purification
存储溶液
Constituents: PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SDF1 beta also known as CXCL12b or stromal cell-derived factor 1 beta is a small chemokine with a mass around 8 kDa. SDF1 beta plays a significant role as a signaling molecule involved in mediating various cellular responses. Expression of this chemokine occurs in a variety of tissues including spleen brain liver and skeletal muscle. Its mechanical function mainly involves binding to the CXCR4 receptor on target cells leading to the activation of signaling cascades that modulate cellular behavior.
Biological function summary

SDF1 beta participates in the regulation of immune cell trafficking and homing. As a prominent chemokine it directs leukocytes to sites where they perform their immunological duties such as inflamed or damaged tissue. Although it generally acts independently SDF1 beta can be part of a larger chemokine network collaborating with other chemokines and cytokines to orchestrate coordinated migration and positioning of immune cells.

Pathways

SDF1 beta is intricately linked with the chemokine signaling pathway and homing of hematopoietic stem cells. Activation of its pathway involves the prominent receptor CXCR4 which mediates signals that are important for the directional movement of these cells. SDF1 beta plays a central role in the hematopoiesis process where it interacts with molecules like CXCR7 which sometimes acts as a supportive player for its receptor influencing the hematopoietic stem cell niche regulation as well.

Involvement of SDF1 beta has been identified in cancer progression and inflammatory diseases like rheumatoid arthritis. In cancer it contributes to tumor growth and metastasis with CXCR4 being its main receptor partner. In rheumatoid arthritis overexpression or dysregulation of SDF1 beta influences the infiltration and retention of immune cells in the joints exacerbating inflammation. Understanding these interactions provides insights for potential therapeutic approaches targeting the SDF1 beta and CXCR4 axis in disease contexts.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils (PubMed : 18802065, PubMed : 39093700). Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis (PubMed : 8752281, PubMed : 18802065, PubMed : 39093700). Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12/SDF-1 (PubMed : 16107333, PubMed : 19255243). Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV : ITGB3, ITGA4 : ITGB1 and ITGA5 : ITGB1 in a CXCR4-independent manner (PubMed : 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase (PubMed : 18802065). Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins (PubMed : 16107333, PubMed : 18802065, PubMed : 19255243, PubMed : 39093700). CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase (PubMed : 18802065). Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1 (PubMed : 8752281). Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation (By similarity). Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).. SDF-1-beta(3-72). Shows a reduced chemotactic activity.. SDF-1-alpha(3-67). Shows a reduced chemotactic activity (PubMed : 14525775). Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites (PubMed : 14525775).
See full target information CXCL12
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 14: PubMed35565857

2022

Piceatannol SNEDDS Attenuates Estradiol-Induced Endometrial Hyperplasia in Rats by Modulation of NF-κB and Nrf2/HO-1 Axes.

Applications

Unspecified application

Species

Unspecified reactive species

Lenah S Binmahfouz,Basma G Eid,Amina M Bagher,Rasheed A Shaik,Najlaa S Binmahfouz,Ashraf B Abdel-Naim
View all publications
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