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Neuroscience Neurotransmission Calcium Signaling Calcium Binding Proteins

生物素Anti-S100抗体(ab48653)

  • Datasheet
  • SDS
Submit a review Q&A (2)

Product price, shipping and contact information

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Abpromise

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Key features and details

  • Biotin Rabbit polyclonal to S100
  • Suitable for: ELISA, EIA, IP, RIA, WB
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

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概述

  • 产品名称

    生物素Anti-S100抗体
    参阅全部 S100 一抗
  • 描述

    生物素兔多克隆抗体to S100
  • 宿主

    Rabbit
  • 偶联物

    Biotin
  • 经测试应用

    适用于: ELISA, EIA, IP, RIA, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length native protein purified from human plasma

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.50
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial

相关产品

  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab48653于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
EIA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.
EIA
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

靶标

  • 功能

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • 组织特异性

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 序列相似性

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P23297 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6271 Human
    • Omim: 176940 Human
    • SwissProt: P23297 Human
    • Unigene: 515715 Human
    • 别名

      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 beta antibody
      • S100 calcium binding protein A1 antibody
      • S100 calcium binding protein B antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S100B antibody
      • S100beta antibody
      • S10A1_HUMAN antibody
      see all

    实验方案

    • Immunoprecipitation protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab48653?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab48653 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    Customer Services and Scientific Support Team,
    Thanks you for your reply. However, there are two issues:
    1. Following the link you provided for product no. ab95190 led me to a
    message saying that the product is no longer available in Abcam's
    product range. Do you have any recommendation as to an alternative for
    the S100 standard protein, for use with ab8330?
    2. It looks like for my application, I will need to amplify my sELISA
    signal using a biotinylated system. I'm considering other antibodies
    in your product range which have not been certified by Abcam for
    sELISA, to use as detection antibody. Are you able to provide a small
    amount of sample antibody for testing, say product no. ab48653 or
    ab105371? I intend to go with ab8330 as capture antibody.
    Many thanks for your help again.
    Best wishes,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2012

    Answer

    Thank you for your reply.

    My sincere apologies for the confusion. In this case, I am sorry we do not have a suitable standard protein to suggest on this occasion.

    In answer to your second question regarding ab48653 and ab105371, I am sorry these have not been tested in sandwich ELISA and regrettably would not be covered by the guarantee for this application. They have been tested only in indirect ELSA for each antibody against its antigen.

    I am sorry to confirm that because we carry over 90 000 products, it is not possible for use tostock sample vials to send to customers. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact me again by replying to this email prior to purchase if you would like more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contactme again with any other questions

    Read More

    Abcam Scientific Support

    回复于 Sep 07 2012

    Question

    Does this antibody recognize S100A or S100B?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 18 2011

    Answer

    Thank you for your phone call last week. I have heard back from my contact at the lab, and I was told that ab48653 recognizes both the A and B forms of S100.  Please let me know if you have further questions.

    Read More

    Abcam Scientific Support

    回复于 Apr 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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