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重组RabMAb

重组生物素Anti-Polyethylene glycol抗体[PEG-B-47b] (ab53449)

Reviews (1)Q&A (5)References (15)
ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
  • Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [PEG-B-47b] to Polyethylene glycol
  • Suitable for: IHC-P, Sandwich ELISA, WB, ELISA
  • Reacts with: Species independent
  • Conjugation: Biotin

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    生物素Anti-Polyethylene glycol抗体[PEG-B-47b]
    参阅全部 Polyethylene glycol 一抗

  • 描述

    生物素兔单克隆抗体[PEG-B-47b] to Polyethylene glycol

  • 宿主

    Rabbit

  • 偶联物

    Biotin

  • 特异性

    ab53449 does not cross react with Tween20.

  • 经测试应用

    适用于: IHC-P, Sandwich ELISA, WB, ELISAmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: BSA-PEG5K.

  • 常规说明

    The smallest PEG molecule which our Polyethylene glycol antibody (ab53449) can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing.

    Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. Our anti-mPEG antibody [PEG-B-47b] reacts to the methoxy group of Polyethylene glycol (PEG). There is no methoxy group in Tween 20, which should not interfere with the function of this antibody. 

    If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 解离常数（KD）

    KD = 2.41 x 10 -9 M
    Learn more about KD

  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    PEG-B-47b

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab53449于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100. Images of positive IHC with this monoclonal antibody, clone PEG-B-47b, are on the ab51257 datasheet.
Sandwich ELISA
Use at an assay dependent concentration.
WB
1/50000. Predicted molecular weight: 2 kDa.

For unpurified use at 1/5000 - 1/20000.
ELISA (1)
Use a concentration of 1 µg/ml.

Coating plates for ELISA: 0 to 500 ng/ml.

For unpurified use at 0.5µg/ml, coating plates for ELISA: 5 to 10 ug/ml.
说明
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100. Images of positive IHC with this monoclonal antibody, clone PEG-B-47b, are on the ab51257 datasheet.
Sandwich ELISA
Use at an assay dependent concentration.
WB
1/50000. Predicted molecular weight: 2 kDa.

For unpurified use at 1/5000 - 1/20000.
ELISA
Use a concentration of 1 µg/ml.

Coating plates for ELISA: 0 to 500 ng/ml.

For unpurified use at 0.5µg/ml, coating plates for ELISA: 5 to 10 ug/ml.

靶标

  • 别名

    • PEG antibody

图片

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.

    Biotin conjugate (ab53449) available for this clone.

  • Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Sandwich ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Sandwich ELISA antigen dose-response curve using purified ab53449.

    Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

    Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

    Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/50000 dilution (purified) + BSA-PEG5K at 0.02 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 2 kDa
    Observed band size: 280 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.

  • ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    ELISA - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

    Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).

  • Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Western blot - Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Lane 1 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/5000 dilution (unpurified)
    Lane 2 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/10000 dilution (unpurified)
    Lane 3 : Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449) at 1/20000 dilution (unpurified)

    All lanes : BSA-PEG

    Predicted band size: 2 kDa

  • Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)
    Biotin Anti-Polyethylene glycol antibody [PEG-B-47b] (ab53449)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (15)

发表研究结果有使用 ab53449？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab53449 被引用在 15 文献中.

  • Sebastiani F  et al. Screening of the binding affinity of serum proteins to lipid nanoparticles in a cell free environment. J Colloid Interface Sci 610:766-774 (2022). PubMed: 34848062
  • Sebastiani F  et al. Apolipoprotein E Binding Drives Structural and Compositional Rearrangement of mRNA-Containing Lipid Nanoparticles. ACS Nano N/A:N/A (2021). PubMed: 33754708
  • van Alem CMA  et al. Liposomal Delivery Improves the Efficacy of Prednisolone to Attenuate Renal Inflammation in a Mouse Model of Acute Renal Allograft Rejection. Transplantation 104:744-753 (2020). PubMed: 31929419
  • Stock AA  et al. Conformal Coating of Stem Cell-Derived Islets for ß Cell Replacement in Type 1 Diabetes. Stem Cell Reports 14:91-104 (2020). PubMed: 31839542
  • Che L  et al. Computer-assisted engineering of programmed drug releasing multilayer nanomedicine via indomethacin-mediated ternary complex for therapy against a multidrug resistant tumor. Acta Biomater N/A:N/A (2019). PubMed: 31344512
View all Publications for this product

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1-6 of 6 Abreviews or Q&A

ELISA abreview for Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin)

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Human Serum (Processed blood sample.)
Specification
Processed blood sample.
Type
Sandwich (Detection)
Blocking step
BSA, Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 20 2014

Question

What size of the branched and linear PEG chain does this antibody react to. Will it react to atleast 500 kDa chain?

Read More
Answer



The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing.

Read More

Question

Is ab53449 purified?

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Answer

Yes, ab53449 is purified before biotinylation. Protein A was used to purify this antibody.

Read More

Question

What is the difference between ab51257 and ab53449?

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Answer

Both antibodies ab51257 and ab53449 are the same clone (PEG-B-47). Product ab53449 is the biotinlyated version of ab51257.

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Question

Which PEG antibody do you recommend in detecting linear PEG? When detecting branched PEG?

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Answer

Both ab51257 and ab53449 detect linear and branched PEG (based on our in-house ELISA results).

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Question

I found in your catalog the following antibodies: ab73909 and ab53449, which I would like to have. Could you please tell me how much these antibodies cost, how long will it take to deliver them? Is it possible to make them without glycerol, BSA and sodium azide?
Best regards

Read More
Answer

Thank you for your email.

Could you let me know how many glycerol, BSA and sodium azide free vials you would like to order? Once we have this information we can then confirm the supply.

Thank you very much for your cooperation. I will look forward to hearing from you soon.

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