生物素Anti-Plasmin抗体(ab48350)
Key features and details
- Biotin Rabbit polyclonal to Plasmin
- Suitable for: ELISA, RIA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-Plasmin抗体 -
描述
生物素兔多克隆抗体to Plasmin -
宿主
Rabbit -
偶联物
Biotin -
特异性
This antibody does recognize plasminogen at about 20-40 %. -
经测试应用
适用于: ELISA, RIA, WBmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) - plasmin purified from human plasma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48350于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
组织特异性
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
疾病相关
Plasminogen deficiency -
序列相似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
结构域
Kringle domains mediate interaction with CSPG4. -
翻译后修饰
N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
细胞定位
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 5340 Human
- Omim: 173350 Human
- SwissProt: P00747 Human
- Unigene: 143436 Human
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别名
- Plasmin light chain B antibody
- Plasminogen antibody
- PLG antibody
- PLMN_HUMAN antibody
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab48350 被引用在 4 文献中.
- Voegeli R et al. Expression and ultrastructural localization of plasmin(ogen) in the terminally differentiated layers of normal human epidermis. Int J Cosmet Sci 41:624-628 (2019). PubMed: 31604367
- Leksa V et al. Dissecting mannose 6-phosphate/insulin-like growth factor 2 receptor complexes that control activation and uptake of plasminogen in cells. J Biol Chem : (2012). WB . PubMed: 22613725
- Barker R et al. Plasminogen and plasmin in Alzheimer's disease. Brain Res 1355:7-15 (2010). WB ; Human . PubMed: 20709033
- Sadagopan S et al. Kaposi's sarcoma-associated herpesvirus upregulates angiogenin during infection of human dermal microvascular endothelial cells, which induces 45S rRNA synthesis, antiapoptosis, cell proliferation, migration, and angiogenesis. J Virol 83:3342-64 (2009). WB ; Human . PubMed: 19158252