生物素Anti-Phosphoserine抗体(ab9335)
Key features and details
- Biotin Rabbit polyclonal to Phosphoserine
- Suitable for: WB, IP, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-Phosphoserine抗体
参阅全部 Phosphoserine 一抗 -
描述
生物素兔多克隆抗体to Phosphoserine -
宿主
Rabbit -
偶联物
Biotin -
特异性
Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. -
经测试应用
适用于: WB, IP, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin. BSA and KLH-phosphoserine conjugates.
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阳性对照
- Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.00
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Immunoaffinity chromatography with phosphoserine-agarose. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 4 µg/ml. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.
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| IP |
Use at an assay dependent dilution.
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| ELISA |
Use a concentration of 0.5 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 4 µg/ml. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. |
|
IP
Use at an assay dependent dilution. |
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ELISA
Use a concentration of 0.5 µg/ml. |
靶标
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相关性
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated protein/peptides, antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family. -
别名
- phospho-Ser antibody
- pS antibody
- pSER antibody
图片
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Western blot - Biotin Anti-Phosphoserine antibody (ab9335)
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
数据表及文件
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Datasheet download
文献 (3)
ab9335 被引用在 3 文献中.
- Zhang C et al. Nuclear accumulation of symplekin promotes cellular proliferation and dedifferentiation in an ERK1/2-dependent manner. Sci Rep 7:3769 (2017). WB ; Human . PubMed: 28630428
- Granovski V et al. Humoral Immune Response in Mice Elicited by Combined Yeast-Derived Hepatitis B Virus Core, Surface, and Mosaic Surface Antigens. Intervirology 60:90-101 (2017). PubMed: 28957812
- Phillips JE et al. Glucocorticoid-induced osteogenesis is negatively regulated by Runx2/Cbfa1 serine phosphorylation. J Cell Sci 119:581-91 (2006). WB . PubMed: 16443755
