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AB271277

生物素Anti-NGF抗体

Biotin Anti-NGF antibody

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Anti-NGF antibody conjugated to Biotin is a rabbit polyclonal antibody suitable for ELISA, Western blot.

查看别名

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

2 Images
ELISA - Biotin Anti-NGF antibody (AB271277)
  • ELISA

Unknown

ELISA - Biotin Anti-NGF antibody (AB271277)

Human NGF was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least XXXX of recombinant NGF protein.

Western blot - Biotin Anti-NGF antibody (AB271277)
  • WB

Supplier Data

Western blot - Biotin Anti-NGF antibody (AB271277)

Lane 1 : Marker.
Lane 2 : Recombinant hNGF at 250ng/lane.
Lane 3 : Recombinant hNGF at 125ng/lane.
Lane 4 : Recombinant hNGF at 62.5ng/lane.
Lane 5 : Recombinant hNGF at 31.25ng/lane.
Lane 6 : Recombinant hNGF at 15.625ng/lane.
Lane 7 : Recombinant hNGF at 7.8ng/lane.
Lane 8 : Recombinant hNGF at 3.9ng/lane.
Lane 9 : Recombinant hNGF at 1.95ng/lane.
Lane 10 : Recombinant hNGF at 0.975ng/lane.
Lane 11 : Recombinant hNGF at 0.4875ng/lane.
Lane 12 : Recombinant hNGF at 0.24ng/lane.

All lanes:

Western blot - Biotin Anti-NGF antibody (ab271277)

Predicted band size: 26 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

偶联物

Biotin

激发波长/发射波长
不含载体蛋白

No

应用

WB, ELISA

applications

免疫原

Recombinant Full Length Protein corresponding to Human NGF.

P01138

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.25-1 µg/mL", "ELISA-species-notes": "<p>Human NGF<strong> </strong>was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271277. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant NGF protein.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p>When used in conjunction with compatible secondary reagents, the detection limit for recombinant human NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.</p>" } } }

性能和储存信息

形式
Lyophilized
复溶
Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
纯化工艺
Affinity purification
存储溶液
Constituents: PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed : 14976160, PubMed : 20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed : 20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed : 20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).
See full target information NGF

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