生物素Anti-Myc tag抗体[Myc.A7] (ab173829)
Key features and details
- Biotin Mouse monoclonal [Myc.A7] to Myc tag
- Suitable for: ICC/IF, IP, ELISA, WB
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
生物素Anti-Myc tag抗体[Myc.A7]
参阅全部 Myc tag 一抗 -
描述
生物素小鼠单克隆抗体[Myc.A7] to Myc tag -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: ICC/IF, IP, ELISA, WBmore details -
免疫原
Synthetic peptide corresponding to Human Myc tag aa 410-419 (C terminal).
Sequence:EQKLISEEDL
Database link: P01106 -
阳性对照
- E. coli lysate containing a multi-epitope tagged protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
Myc.A7 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173829于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/500 - 1/2000.
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Predicted molecular weight: 1.2 kDa.
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说明 |
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ICC/IF
1/500 - 1/2000. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 1.2 kDa. |
靶标
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相关性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
细胞定位
Nuclear -
别名
- c-myc tag antibody
- Myc Epitope Tag antibody
图片
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All lanes : Biotin Anti-Myc tag antibody [Myc.A7] (ab173829) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
Secondary
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 1.2 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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