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Tags & Cell Markers Epitope Tags Myc Tag

生物素Anti-Myc tag抗体[9E10] - C-terminal (ab197139)

  • Datasheet
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Key features and details

  • Biotin Mouse monoclonal [9E10] to Myc tag - C-terminal
  • Suitable for: Flow Cyt, Flow Cyt (Intra)
  • Conjugation: Biotin
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC HRP PE SureLight® APC Unconjugated

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概述

  • 产品名称

    生物素Anti-Myc tag抗体[9E10] - C-terminal
    参阅全部 Myc tag 一抗
  • 描述

    生物素小鼠单克隆抗体[9E10] to Myc tag - C-terminal
  • 宿主

    Mouse
  • 偶联物

    Biotin
  • 特异性

    This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc.
  • 经测试应用

    适用于: Flow Cyt, Flow Cyt (Intra)more details
  • 免疫原

    Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal).
    Database link: P01106

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • 存储溶液

    pH: 7.40
    Preservative: 0.0975% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度

    Size exclusion
  • 纯化说明

    Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
  • 克隆

    单克隆
  • 克隆编号

    9E10
  • 同种型

    IgG1
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
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    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

相关产品

  • Alternative Versions

    • DyLight® 650 Anti-Myc tag antibody [9E10] (ab117487)
    • DyLight® 488 Anti-Myc tag antibody [9E10] (ab117499)
    • DyLight® 550 Anti-Myc tag antibody [9E10] (ab117511)
    • FITC Anti-Myc tag antibody [9E10] (ab117599)
    • Alexa Fluor® 488 Anti-Myc tag antibody [9E10] (ab202008)
    • Anti-Myc tag antibody [9E10] (ab206486)
    • Alexa Fluor® 594 Anti-Myc tag antibody [9E10] (ab223894)
    • Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
    • APC Anti-Myc tag antibody [9E10] (ab223896)
    • Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
    • Anti-Myc tag antibody [9E10] (ab32)
    • HRP Anti-Myc tag antibody [9E10] (ab62928)
    • PE Anti-Myc tag antibody [9E10] (ab72468)
    • SureLight® APC Anti-Myc tag antibody [9E10] (ab72580)
    • Biotin Anti-Myc tag antibody [9E10] (ab81658)
  • Isotype control

    • Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)
  • Related Products

    • Recombinant human c-Myc protein (Active) (ab169901)
    • Recombinant Human c-Myc protein (ab84132)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab197139于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use a concentration of 1 - 5 µg/ml.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

说明
Flow Cyt
Use a concentration of 1 - 5 µg/ml.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位

    Nuclear
  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

实验方案

  • Flow cytometry protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab197139?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab197139 被引用在 1 文献中.

  • Xia F  et al. Genome-wide in vivo screen of circulating tumor cells identifies SLIT2 as a regulator of metastasis. Sci Adv 8:eabo7792 (2022). PubMed: 36054348

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