AB271250

生物素Anti-MCP1抗体

Name: Biotin Anti-MCP1抗体 (ab271250)| Abcam中文官网
Brand: Abcam Limited
SKU: AB271250
Price: 4815 CNY
Availability: InStock

Biotin Anti-MCP1 antibody

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Rabbit Polyclonal MCP1 antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCL2 aa 1 to C-terminus.

查看别名

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

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ELISA - Biotin Anti-MCP1 antibody (AB271250)

ELISA

Unknown

ELISA - Biotin Anti-MCP1 antibody (AB271250)

Human MCP1 was detected by sandwich ELISA (using 100 μl/well) using a concentration of 0.25-1.0 μg/ml of ab271250. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant MCP1 protein.

Supplier Data

Western blot - Biotin Anti-MCP1 antibody (AB271250)

Western blot analysis of varying levels of Recombinant human MCP-1 protein using ab271250 at 0.1 μg/ml.

Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

All lanes:

Western blot - Biotin Anti-MCP1 antibody (ab271250)

Predicted band size: 11 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

偶联物

Biotin

激发波长/发射波长

不含载体蛋白

应用

ELISA, WB

applications

免疫原

Recombinant Fragment Protein within Human CCL2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13500

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.25-1 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.2 µg/mL", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Lyophilized

复溶

Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.

纯化工艺

Affinity purification

存储溶液

Constituents: PBS, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).

See full target information CCL2

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生物素Anti-MCP1抗体

Biotin Anti-MCP1 antibody

ELISA - Biotin Anti-MCP1 antibody (AB271250)

Western blot - Biotin Anti-MCP1 antibody (AB271250)

关键信息

宿主种属

克隆

亚型

偶联物

激发波长/发射波长

不含载体蛋白

应用

免疫原