生物素Anti-M13抗体[E1] (ab17269)
![ELISA - Biotin Anti-M13 antibody [E1] (ab17269)](https://www.abcam.cn/ps/products/17/ab17269/Images/ab17269-459706-biotin-anti-m13-antibody-e1-elisa.jpg "ELISA - Biotin Anti-M13 antibody [E1] (ab17269)")
Key features and details
- Biotin Mouse monoclonal [E1] to M13
- Suitable for: WB, ELISA, Flow Cyt, IHC
- Conjugation: Biotin
- Isotype: IgG3
概述
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产品名称
生物素Anti-M13抗体[E1]
参阅全部 M13 一抗 -
描述
生物素小鼠单克隆抗体[E1] to M13 -
宿主
Mouse -
偶联物
Biotin -
特异性
M13 antibody [E1] (ab17269) is specific for gp3 protein. -
经测试应用
适用于: WB, ELISA, Flow Cyt, IHCmore details -
种属反应性
与反应: Enterobacteria phage M13 -
免疫原
Full length M13 phage coat protein; Exact epitope not determined.
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常规说明
M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
M13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter. -
Primary antibody说明
M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries. -
克隆
单克隆 -
克隆编号
E1 -
骨髓瘤
unknown -
同种型
IgG3 -
轻链类型
unknown -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml.
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|
| ELISA |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use 1µg for 106 cells.
ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| IHC |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. |
|
ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1µg for 106 cells. ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IHC
Use at an assay dependent concentration. |
靶标
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相关性
The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle. -
别名
- Bacteriophage M13 antibody
- Coliphage M13 antibody
- Enterobacteria phage M13 antibody
see all
图片
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ELISA - Biotin Anti-M13 antibody [E1] (ab17269)
ELISA absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of ab17269.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab17269 尚未被引用在任何文献中。