生物素Anti-Listeria monocytogenes抗体(ab20766)
Key features and details
- Biotin Rabbit polyclonal to Listeria monocytogenes
- Suitable for: ELISA, IHC-Fr
- Reacts with: Listeria monocytogenes
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-Listeria monocytogenes抗体
参阅全部 Listeria monocytogenes 一抗 -
描述
生物素兔多克隆抗体to Listeria monocytogenes -
宿主
Rabbit -
偶联物
Biotin -
经测试应用
适用于: ELISA, IHC-Frmore details -
种属反应性
与反应: Listeria monocytogenes -
免疫原
Tissue, cells or virus corresponding to Listeria monocytogenes Listeria monocytogenes.
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常规说明
IgG fraction covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
IgG fraction -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20766于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/1000.
|
|
| IHC-Fr | (1) |
1/250.
|
| 说明 |
|---|
|
ELISA
1/1000. |
|
IHC-Fr
1/250. |
靶标
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相关性
The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection. -
数据库链接
- Entrez Gene: 2831193 Listeria monocytogenes
- SwissProt: C1L1U9 Listeria monocytogenes
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别名
- L. monocytogenes antibody
图片
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Immunohistochemistry (Frozen sections) - Biotin Anti-Listeria monocytogenes antibody (ab20766)This image is a courtesy of Anonymous Abreview
ab20766 staining Listeria monocytogenes in mouse spleen tissue section from a mice infected with 1x10exp6 L. monocytogenes for 24h by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 4% paraformaldehyde for 2 hours at RT and then it was incubated ON in 30% Sucrose in PBS at 4°C, froze away in OCT/Tissuetek. The tissue samples were blocked with 1% 1:20 Cd16/32 at 200C for 30 minutes. The sample was incubated with primary antibody (1/250) in PBS at 200C for 1 hour. For detection Streptavidin-Cy3 was used at dilution at 1:500 in PBS for 30 min at 20°C.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab20766 被引用在 1 文献中.
- Dresing P et al. A fluorescence reporter model defines "Tip-DCs" as the cellular source of interferon ß in murine listeriosis. PLoS One 5:e15567 (2010). IHC-Fr . PubMed: 21179567