重组生物素Anti-Lipocalin-2 / NGAL [EPR21092] (ab318314)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Lipocalin-2 / NGAL [EPR21092] (ab318314)](https://www.abcam.cn/ps/products/318/ab318314/Images/ab318314-1-ab318314-AntiLipocalin-2-NGAL-antibody-EPR21092-Biotin-spleen-mouse.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Lipocalin-2 / NGAL [EPR21092] (ab318314)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [EPR21092] to Lipocalin-2 / NGAL
- Suitable for: IHC-P
- Reacts with: Mouse
- Conjugation: Biotin
Related conjugates and formulations
概述
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产品名称
生物素Anti-Lipocalin-2 / NGAL [EPR21092]
参阅全部 Lipocalin-2 / NGAL 一抗 -
描述
生物素兔单克隆抗体[EPR21092] to Lipocalin-2 / NGAL -
宿主
Rabbit -
偶联物
Biotin -
特异性
This antibody is not recommended for use in Cell Samples in Western Blot as it detected a strong nonspecific band close to the NGAL position.
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经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P : Mouse spleen
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21092 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Immunohistochemistry kits
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318314于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
组织特异性
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
序列相似性
Belongs to the calycin superfamily. Lipocalin family. -
细胞定位
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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数据库链接
- Entrez Gene: 16819 Mouse
- SwissProt: P11672 Mouse
- Unigene: 9537 Mouse
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别名
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Lipocalin-2 / NGAL [EPR21092] (ab318314)
IHC image of Lipocalin-2 staining in a section of formalin-fixed paraffin-embedded mouse spleen.
Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab318314 at 2 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Lipocalin-2 / NGAL [EPR21092] (ab318314)](https://www.abcam.cn/ps/products/318/ab318314/Images/ab318314-2-ab318314-Anti-Lipocalin-2-NGAL-antibody-EPR21092-Biotin-negative-brain-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Lipocalin-2 / NGAL [EPR21092] (ab318314)Negative staining of Lipocalin-2 in a section of formalin-fixed paraffin-embedded mouse normal brain.
Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab318314 at 2 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Biotin Anti-Lipocalin-2 / NGAL [EPR21092] (ab318314)](https://www.abcam.cn/ps/products/318/ab318314/Images/ab318314-3-benefitsofrecombinantantibodies.jpg)
Biotin Anti-Lipocalin-2 / NGAL [EPR21092] (ab318314)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab318314 尚未被引用在任何文献中。