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生物素Anti-IRE1 (phospho S724)抗体(ab81936)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IRE1 (phospho S724) antibody (ab81936)

    Key features and details

    • Biotin Rabbit polyclonal to IRE1 (phospho S724)
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat, Human
    • Conjugation: Biotin
    • Isotype: IgG

    概述

    • 产品名称

      生物素Anti-IRE1 (phospho S724)抗体
      参阅全部 IRE1 一抗

    • 描述

      生物素兔多克隆抗体to IRE1 (phospho S724)

    • 宿主

      Rabbit

    • 偶联物

      Biotin

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Mouse, Rat, Human
      不与反应: Monkey

    • 免疫原

      Synthetic peptide corresponding to Human IRE1 (phospho S724).

    • 阳性对照

      • Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells and insuli. COS-7 expressing wild-type IRE1. Min6 cells treated with glucose.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Constituents: 30% Glycerol, PBS
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab81936于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/300.
    说明
    IHC-P
    1/300.

    靶标

    • 功能

      Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

    • 组织特异性

      Ubiquitously expressed. High levels observed in pancreatic tissue.

    • 序列相似性

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 KEN domain.
      Contains 1 protein kinase domain.

    • 翻译后修饰

      Autophosphorylated.

    • 细胞定位

      Endoplasmic reticulum membrane.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IRE1 (phospho S724) antibody (ab81936)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-IRE1 (phospho S724) antibody (ab81936)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling IRE1 with the unconjugated version of at81936 at 1/300 dilution. 

    文献 (1)

    发表研究结果有使用 ab81936？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab81936 被引用在 1 文献中.

    • He Y  et al. Palmitic acid, but not high-glucose, induced myocardial apoptosis is alleviated by N-acetylcysteine due to attenuated mitochondrial-derived ROS accumulation-induced endoplasmic reticulum stress. Cell Death Dis 9:568 (2018). PubMed: 29752433

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IRE1 (phospho S724) antibody (Biotin)

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (AR42J)
    Permeabilization
    Yes - 10% Triton x100
    Specification
    AR42J
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 24 2017

    Question

    Would like to try this antibody with rat samples.

    Read More
    Answer

    Thank you very much for your call today.
    DISCOUNT CODE: ***
    Expiration date: December 14, 2012
    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab104157 with rat samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

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