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AB193185

Biotin Anti-Human Ig light chain 抗体 [RM129]

Biotin Anti-Human Ig light chain antibody [RM129]

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(1 Publication)

Rabbit Recombinant Monoclonal IGKC antibody - conjugated to Biotin. Suitable for ELISA, sELISA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human IGKC.

查看别名

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

3 Images
Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

A titer Sandwich ELISA using ab193185 as the detection antibody. The plate, coated with the capture antibody anti-human IgG, was loaded with different amounts of human IgG. A serial dilution of ab193185 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.

Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)
  • sELISA

Supplier Data

Sandwich ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

Sandwich ELISA using ab195581 as the capture antibody (100 ng/well), and Biotin Anti-Human Ig light chain antibody ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)
  • ELISA

Supplier Data

ELISA - Biotin Anti-Human Ig light chain antibody [RM129] (AB193185)

ELISA of human immunoglobulins shows ab193185 reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain. The plate was coated with 50ng/well of different immunglobulins. 100 ng/mL or 20 ng/mL was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

RM129

亚型

IgG

偶联物

Biotin

激发波长/发射波长
不含载体蛋白

No

反应种属

Human

应用

sELISA, ELISA

applications

免疫原

Full Length Protein corresponding to Human IGKC. The exact immunogen used to generate this antibody is proprietary information.

P01834

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.02-0.25 µg/mL", "sELISA-species-notes": "<p>May be paired with <a href='/products/primary-antibodies/human-igg-antibody-rm116-ab195574'>ab195574</a>.</p>" }, "Mouse": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "0.02-0.25 µg/mL", "sELISA-species-notes": "<p>May be paired with <a href='/products/primary-antibodies/human-igg-antibody-rm116-ab195574'>ab195574</a>.</p>" }, "Rat": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "0.02-0.25 µg/mL", "sELISA-species-notes": "<p>May be paired with <a href='/products/primary-antibodies/human-igg-antibody-rm116-ab195574'>ab195574</a>.</p>" }, "Goat": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "0.02-0.25 µg/mL", "sELISA-species-notes": "<p>May be paired with <a href='/products/primary-antibodies/human-igg-antibody-rm116-ab195574'>ab195574</a>.</p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
Protein A affinity purified from an animal origin–free culture supernatant.
存储溶液
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle|Store in the dark
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The human immunoglobulin light chain often referred to as "Ig light chain" or "IgL" is a component of antibodies. It's an essential part of the adaptive immune system. The light chains combine with heavy chains to form the complete immunoglobulin molecule. It has approximately 25 kDa mass. The light chain can be kappa (κ) or lambda (λ) types depending on the sequence. Expression occurs mainly in B lymphocytes where it plays a critical role in antibody diversity and specificity.
Biological function summary

The light chain contributes to antigen recognition by forming part of the antigen-binding site on an antibody. It's not a standalone entity but part of a larger immunoglobulin complex. This allows antibodies to bind specifically to antigens facilitating the immune response. The light chain also participates in stabilizing the antibody structure and ensuring proper immune function.

Pathways

The Ig light chain functions in the context of the adaptive immune system particularly in the immunoglobulin gene rearrangement and affinity maturation pathways. It works closely with the heavy chain to achieve effective antigen recognition. Additionally it interacts indirectly with proteins like B-cell receptor (BCR) which is important in the B lymphocyte signaling pathway. These interactions allow for the antibody-mediated detection and response to pathogens.

Abnormal Ig light chain production correlates with multiple myeloma and light chain amyloidosis. In multiple myeloma malignant plasma cells overproduce light chains often leading to their deposition in tissues and subsequent organ damage. In light chain amyloidosis the excess light chains misfold and deposit as amyloid fibrils causing organ dysfunction. Associations between these conditions and other immunoglobulin proteins highlight the role of Ig light chains in pathological processes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).
See full target information IGKC
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Allergy, asthma, and clinical immunology : officia 16:30 PubMed32351585

2020

Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients.

Applications

Unspecified application

Species

Unspecified reactive species

Hossein Aazami,Farhad Seif,Babak Ghalehbaghi,Pegah Babaheidarian,Alireza Mohebbi,Aslan Ahmadi,Majid Khoshmirsafa,Sahand Ghalehbaghi,Babak Behnam,Kobra Zinat Entezami,Zahra Madjd,Reza Falak
View all publications
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替代产品

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搭配使用产品

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Secondary Antibodies

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Secondary Antibodies

AB125919

Rabbit monoclonal [H19-5] Anti-Human kappa light chain

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Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

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