生物素Anti-HLA G抗体[4H84] (ab52456)
![Western blot - Biotin Anti-HLA G antibody [4H84] (ab52456)](https://www.abcam.cn/ps/products/52/ab52456/Images/ab52456-522213-biotin-antihla-g-antibody-western-blot-human-2.jpg "Western blot - Biotin Anti-HLA G antibody [4H84] (ab52456)")
Key features and details
- Biotin Mouse monoclonal [4H84] to HLA G
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
生物素Anti-HLA G抗体[4H84]
参阅全部 HLA G 一抗 -
描述
生物素小鼠单克隆抗体[4H84] to HLA G -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human HLA G aa 61-83.
Sequence:DSDSACPRMEPRAPWVEQEGPEY
Database link: P17693 -
阳性对照
- WB: HLA-G1 transfectants (LCL-HLA-G1).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Prior to conjugation, ab52456 was purified by Protein A affinity chromatography. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
4H84 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52456于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). |
靶标
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功能
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
组织特异性
Expressed in trophoblasts. -
序列相似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
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别名
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
图片
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Western blot - Biotin Anti-HLA G antibody [4H84] (ab52456)All lanes : Biotin Anti-HLA G antibody [4H84] (ab52456) at 2 µg/ml
Lane 1 : JEG-3 red
Lane 2 : LNCaP red
Lane 3 : JEG-3 non-red
Lane 4 : LNCap non-red
Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?Following biotinylated mouse anti-HLA-G monoclonal antibody (ab52456) incubation, IRDye800-conjugated streptavidin was added to the nitrocellulose membrane.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab52456 被引用在 1 文献中.
- Moreau P et al. HLA-G gene repression is reversed by demethylation. Proc Natl Acad Sci U S A 100:1191-6 (2003). WB, ICC ; Human . PubMed: 12552087