生物素抗丙型肝炎病毒抗体[NS5B-6] (ab122972)
![Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)](https://www.abcam.cn/ps/products/122/ab122972/Images/Hepatitis-C-Virus-Primary-antibodies-ab122972-2.jpg "Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)")
Key features and details
- Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: Flow Cyt (Intra), WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2b
概述
-
产品名称
生物素抗丙型肝炎病毒抗体[NS5B-6]
参阅全部 Hepatitis C Virus 一抗 -
描述
生物素小鼠单克隆抗体[NS5B-6] to丙型肝炎病毒 -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: Flow Cyt (Intra), WB, ELISA, ICC/IFmore details -
种属反应性
与反应: Hepatitis C virus -
免疫原
Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli
-
阳性对照
- Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
-
常规说明
Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
filter-sterilized -
克隆
单克隆 -
克隆编号
NS5B-6 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122972于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa).
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa). |
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
-
相关性
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. -
细胞定位
Virus surface -
数据库链接
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P27958 Hepatitis C virus
-
别名
- Genome polyprotein antibody
- HCV antibody
- Hepatitis C virus polyprotein antibody
图片
-
Western blot - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972) + Lysate of chimp liver cells infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 70 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
NS5b protein is detected as a 58kDa band. -
![Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)](https://www.abcam.cn/ps/products/122/ab122972/Images/Hepatitis-C-Virus-Primary-antibodies-ab122972-3.jpg)
Immunocytochemistry/ Immunofluorescence - Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972)ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab122972 被引用在 3 文献中.
- Zhang MQ et al. A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells. Autophagy 16:2167-2179 (2020). PubMed: 31996071
- Zhang MQ et al. Differential Effects of Autophagy-Related 10 Protein on HCV Replication and Autophagy Flux Are Mediated by Its Cysteine44 and Cysteine135. Front Immunol 9:2176 (2018). PubMed: 30319633
- Manabe S et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245