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Tags & Cell Markers Epitope Tags HA Tag

生物素Anti-HA tag抗体(ab26228)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (18)

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Key features and details

  • Biotin Rabbit polyclonal to HA tag
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgG

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概述

  • 产品名称

    生物素Anti-HA tag抗体
    参阅全部 HA tag 一抗
  • 描述

    生物素兔多克隆抗体to HA tag
  • 宿主

    Rabbit
  • 偶联物

    Biotin
  • 经测试应用

    适用于: WB, ELISAmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    HA-tag conjugated to KLH

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: HEPES, 0.2% BSA
  • Concentration information loading...
  • 纯化说明

    Immunoaffinity purified using the peptide conjugated to a solid-phase support
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag

相关产品

  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab26228于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/5000.
ELISA
1/5000 - 1/25000.
说明
WB
1/1000 - 1/5000.
ELISA
1/5000 - 1/25000.

靶标

  • 相关性

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 别名

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (18)

发表研究结果有使用 ab26228?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab26228 被引用在 18 文献中.

  • Habrian C  et al. Homo- and hetero-dimeric subunit interactions set affinity and efficacy in metabotropic glutamate receptors. Nat Commun 14:8288 (2023). PubMed: 38092773
  • Lee J  et al. Distinct beta-arrestin coupling and intracellular trafficking of metabotropic glutamate receptor homo- and heterodimers. Sci Adv 9:eadi8076 (2023). PubMed: 38055809
  • Hobson BD  et al. Subcellular and regional localization of mRNA translation in midbrain dopamine neurons. Cell Rep 38:110208 (2022). PubMed: 35021090
  • Dirscherl C  et al. Dissociation of β2m from MHC class I triggers formation of noncovalent transient heavy chain dimers. J Cell Sci 135:N/A (2022). PubMed: 35393611
  • Thibado JK  et al. Differences in interactions between transmembrane domains tune the activation of metabotropic glutamate receptors. Elife 10:N/A (2021). PubMed: 33880992
View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Flow Cytometry abreview for Anti-HA tag antibody (Biotin)

Good
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (A3.01 human CD4+ T cell line engineered to express)
Permeabilization
No
Gating Strategy
Gated on major population by FSC/SSC (i.e. excluding dead cells/debris)
Specification
A3.01 human CD4+ T cell line engineered to express
Preparation
Cell harvesting/tissue preparation method: Suspension cells
Sample buffer: RPMI 1640/10% FBS
Fixation
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Menelaos Symeonides

Verified customer

提交于 Mar 09 2020

Question

 Your product ab26228 is a biotinylated rabbit anti-HA polyclonal. Is that derived from the same rabbit anti-HA polyclonal as in ab9110?

Read More

Abcam community

Verified customer

Asked on Nov 17 2011

Answer

Yes, ab9110 is the unconjugated form of ab26228 (biotinylated form).

Read More

Abcam Scientific Support

回复于 Nov 17 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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