生物素Anti-GST抗体(ab71283)
Key features and details
- Biotin Goat polyclonal to GST
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-GST抗体
参阅全部 GST 一抗 -
描述
生物素山羊多克隆抗体to GST -
宿主
Goat -
偶联物
Biotin -
经测试应用
适用于: ELISA, WB, IHC-Pmore details -
种属反应性
与反应: Species independent -
免疫原
Glutathione-S-Transferase [Schistosoma japonicum]
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常规说明
The antibody was labelled with Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Anti-GST Antibody, Biotin Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71283于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
IHC-P
Use at an assay dependent concentration. |
靶标
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相关性
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. -
别名
- Glutathione S Transferase antibody
- Glutathione S-transferase class-mu 26 kDa isozyme antibody
- GST antibody
see all
图片
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ELISA results of ab71283 tested against purified GST. Each well was coated in duplicate with 1.0 µg of GST. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Streptavidin Peroxidase Conjugated and TMB substrate.
数据表及文件
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Datasheet download
文献 (1)
ab71283 被引用在 1 文献中.
- Rockel S et al. iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks. Nucleic Acids Res 41:e52 (2013). PubMed: 23258699