AB323677

Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915]

BOND RX™ Validated
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Glucose Transporter GLUT1 antibody - conjugated to Biotin. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

GLUT1, SLC2A1, HepG2 glucose transporter, GLUT-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB323677)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB323677)

Low expression staining of SLC2A1 in a section of formalin-fixed paraffin-embedded human normal pancreas*.

Performed on a Leica BOND^TM system. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab323677 at 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB323677)

IHC image of SLC2A1 staining in a section of formalin-fixed paraffin-embedded human normal liver*.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

不同偶联物与剂型 (11)

Unconjugated

Anti-Glucose Transporter GLUT1 antibody [EPR3915]
Carrier free

Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free
Carrier free

Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free
660 APC

APC Anti-Glucose Transporter GLUT1 antibody [EPR3915]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
519 FITC

FITC Anti-Glucose Transporter GLUT1 antibody [EPR3915]
HRP

HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915]
578 PE

PE Anti-Glucose Transporter GLUT1 antibody [EPR3915]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3915

亚型

IgG

偶联物

Biotin

激发波长/发射波长

不含载体蛋白

反应种属

Human, Human, Mouse, Rat

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

We recommend not to boil the samples after lysis to get desired WB bands.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.

Biological function summary

This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.

Pathways

GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.

GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed : 10227690, PubMed : 10954735, PubMed : 18245775, PubMed : 19449892, PubMed : 25982116, PubMed : 27078104, PubMed : 32860739). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed : 18245775, PubMed : 19449892). Most important energy carrier of the brain : present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed : 10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity). Required for mesendoderm differentiation (By similarity).

See full target information SLC2A1

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915]