生物素Anti-Fluorescein抗体(ab6655)
Key features and details
- Biotin Goat polyclonal to Fluorescein
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-Fluorescein抗体
参阅全部 Fluorescein 一抗 -
描述
生物素山羊多克隆抗体to Fluorescein -
宿主
Goat -
偶联物
Biotin -
经测试应用
适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Fluorescein conjugated to bovine serum albumin.
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常规说明
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| IHC-P |
1/1000 - 1/5000. PubMed: 26838601
|
|
| IHC-Fr |
1/1000 - 1/5000.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| In situ hybridization |
Use at an assay dependent concentration.
|
|
| WB |
1/2000 - 1/10000.
|
| 说明 |
|---|
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
|
IHC-P
1/1000 - 1/5000. PubMed: 26838601 |
|
IHC-Fr
1/1000 - 1/5000. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
In situ hybridization
Use at an assay dependent concentration. |
|
WB
1/2000 - 1/10000. |
靶标
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相关性
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
别名
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
图片
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Western blot - Biotin Anti-Fluorescein antibody (ab6655)Biotin Anti-Fluorescein antibody (ab6655) at 1/1000 dilution + BSA conjugated Fluorescein.
Secondary
Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (ab6655)Cropped image from Long et al, Nature Communications, 7, 10354, Fig. 7a.; doi: 10.1038/ncomms10354 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab6655 被引用在 3 文献中.
- Melms JC et al. A molecular single-cell lung atlas of lethal COVID-19. Nature 595:114-119 (2021). PubMed: 33915568
- He B et al. Vascular targeting of LIGHT normalizes blood vessels in primary brain cancer and induces intratumoural high endothelial venules. J Pathol 245:209-221 (2018). PubMed: 29603739
- Long K et al. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun 7:10354 (2016). IHC-Fr, IHC-FrFl . PubMed: 26838601