JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB6655

Biotin Anti-Fluorescein 抗体

Biotin Anti-Fluorescein antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Anti-Fluorescein antibody conjugated to Biotin is a goat polyclonal antibody suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, ISH, Western blot.

查看别名

FI, fluorescein isothyocianate, fluorescyl

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (AB6655)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (AB6655)

Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.

Cropped image from Long et al, Nature Communications, 7, 10354, Fig. 7a.; doi: 10.1038/ncomms10354 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Western blot - Biotin Anti-Fluorescein antibody (AB6655)
  • WB

Supplier Data

Western blot - Biotin Anti-Fluorescein antibody (AB6655)

All lanes:

Western blot - Biotin Anti-Fluorescein antibody (ab6655) at 1/1000 dilution

All lanes:

BSA conjugated Fluorescein.

Secondary

All lanes:

Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

偶联物

Biotin

激发波长/发射波长
不含载体蛋白

No

应用

ISH, WB, Dot, IM, IHC-P, ELISA, IHC-Fr

applications

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IM" : {"fullname" : "Immunomicroscopy", "shortname":"IM"}, "ISH" : {"fullname" : "In Situ Hybridisation", "shortname":"ISH"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/10000 - 1/50000", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IM-species-checked": "guaranteed", "IM-species-dilution-info": "", "IM-species-notes": "<p></p>", "ISH-species-checked": "guaranteed", "ISH-species-dilution-info": "", "ISH-species-notes": "<p></p>" } } }

产品详情

Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
Fluorescein Antibody Biotin Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
存储溶液
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle|Store in the dark

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature 610:356-365 PubMed36198802

2022

Opposing roles of hepatic stellate cell subpopulations in hepatocarcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Aveline Filliol,Yoshinobu Saito,Ajay Nair,Dianne H Dapito,Le-Xing Yu,Aashreya Ravichandra,Sonakshi Bhattacharjee,Silvia Affo,Naoto Fujiwara,Hua Su,Qiuyan Sun,Thomas M Savage,John R Wilson-Kanamori,Jorge M Caviglia,LiKang Chin,Dongning Chen,Xiaobo Wang,Stefano Caruso,Jin Ku Kang,Amit Dipak Amin,Sebastian Wallace,Ross Dobie,Deqi Yin,Oscar M Rodriguez-Fiallos,Chuan Yin,Adam Mehal,Benjamin Izar,Richard A Friedman,Rebecca G Wells,Utpal B Pajvani,Yujin Hoshida,Helen E Remotti,Nicholas Arpaia,Jessica Zucman-Rossi,Michael Karin,Neil C Henderson,Ira Tabas,Robert F Schwabe

Nature 595:114-119 PubMed33915568

2021

A molecular single-cell lung atlas of lethal COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes C Melms,Jana Biermann,Huachao Huang,Yiping Wang,Ajay Nair,Somnath Tagore,Igor Katsyv,André F Rendeiro,Amit Dipak Amin,Denis Schapiro,Chris J Frangieh,Adrienne M Luoma,Aveline Filliol,Yinshan Fang,Hiranmayi Ravichandran,Mariano G Clausi,George A Alba,Meri Rogava,Sean W Chen,Patricia Ho,Daniel T Montoro,Adam E Kornberg,Arnold S Han,Mathieu F Bakhoum,Niroshana Anandasabapathy,Mayte Suárez-Fariñas,Samuel F Bakhoum,Yaron Bram,Alain Borczuk,Xinzheng V Guo,Jay H Lefkowitch,Charles Marboe,Stephen M Lagana,Armando Del Portillo,Emily J Tsai,Emmanuel Zorn,Glen S Markowitz,Robert F Schwabe,Robert E Schwartz,Olivier Elemento,Anjali Saqi,Hanina Hibshoosh,Jianwen Que,Benjamin Izar

Cell reports 30:714-724.e5 PubMed31968248

2020

Remodeling of Metastatic Vasculature Reduces Lung Colonization and Sensitizes Overt Metastases to Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo He,Anna Johansson-Percival,Joseph Backhouse,Ji Li,Gabriel Yin Foo Lee,Juliana Hamzah,Ruth Ganss

The Journal of pathology 245:209-221 PubMed29603739

2018

Vascular targeting of LIGHT normalizes blood vessels in primary brain cancer and induces intratumoural high endothelial venules.

Applications

Unspecified application

Species

Unspecified reactive species

Bo He,Arnaud Jabouille,Veronica Steri,Anna Johansson-Percival,Iacovos P Michael,Venkata Ramana Kotamraju,Reimar Junckerstorff,Anna K Nowak,Juliana Hamzah,Gabriel Lee,Gabriele Bergers,Ruth Ganss

Nature communications 7:10354 PubMed26838601

2016

Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin.

Applications

IHC-FrFl, IHC-Fr

Species

Unspecified reactive species, Unspecified reactive species

K Long,L Moss,L Laursen,L Boulter,C Ffrench-Constant
View all publications

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂,为您科研的每一步提供支持。若我们的产品未能达到预期性能,我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com