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生物素Anti-Cytokeratin 18抗体[DC-10] (ab27551)

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Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551)
  • Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551)

Key features and details

  • Biotin Mouse monoclonal [DC-10] to Cytokeratin 18
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

概述

  • 产品名称

    生物素Anti-Cytokeratin 18抗体[DC-10]
    参阅全部 Cytokeratin 18 一抗

  • 描述

    生物素小鼠单克隆抗体[DC-10] to Cytokeratin 18

  • 宿主

    Mouse

  • 偶联物

    Biotin

  • 特异性

    ab27551 recognises cytokeratin peptide 18 (45 kDa) in human tissue.

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human Cytokeratin 18. Human breast carcinoma cell line PMC-42

  • 阳性对照

    • Flow Cyt (Intra): HeLa human cervix carcinoma cell. WB: HT-29 (reducing and non-reducing conditions) cell lysate.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab27551于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use a concentration of 2 - 6 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.
说明
Flow Cyt (Intra)
Use a concentration of 2 - 6 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 48 kDa.

靶标

  • 功能

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

  • 组织特异性

    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

  • 疾病相关

    Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

  • 序列相似性

    Belongs to the intermediate filament family.

  • 翻译后修饰

    Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

  • 细胞定位

    Cytoplasm > perinuclear region.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Cell proliferation inducing gene 46 protein antibody
      • Cell proliferation inducing protein 46 antibody
      • Cell proliferation-inducing gene 46 protein antibody
      • CK 18 antibody
      • CK-18 antibody
      • CK18 antibody
      • CYK 18 antibody
      • CYK18 antibody
      • Cytokeratin 18 antibody
      • Cytokeratin endo B antibody
      • Cytokeratin-18 antibody
      • K 18 antibody
      • K18 antibody
      • K1C18_HUMAN antibody
      • KA18 antibody
      • Keratin 18 antibody
      • Keratin 18, type I antibody
      • Keratin D antibody
      • keratin, type I cytoskeletal 18 antibody
      • Keratin-18 antibody
      • Krt18 antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551)
      Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551)

      Flow Cytometry analysis (intracellular staining) of HeLa cells labeling Cytokeratin 18 with ab27551 and streptavidin APC at 6 µg / ml concentration (red), and blank sample (black)

    • Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551)
      Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551)
      All lanes : Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551) at 2 µg/ml

      Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)
      Lanes 2-3 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (reducing conditions)
      Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)

      Predicted band size: 48 kDa
      Observed band size: 46 kDa why is the actual band size different from the predicted?

    文献 (0)

    发表研究结果有使用 ab27551？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab27551 尚未被引用在任何文献中。

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