AB27551

生物素Anti-Cytokeratin 18抗体[DC-10]

Name: Biotin Anti-Cytokeratin 18抗体[DC-10] (ab27551)| Abcam中文官网
Brand: Abcam Limited
SKU: AB27551
Price: 4695 CNY
Availability: InStock

Biotin Anti-Cytokeratin 18 antibody [DC-10]

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(1 Publication)

Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Biotin. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing KRT18 protein.

查看别名

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

2 Images

Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

Flow Cytometry analysis (intracellular staining) of HeLa cells labeling Cytokeratin 18 with ab27551 and streptavidin APC at 6 μg / ml concentration (red), and blank sample (black)

Supplier Data

Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

All lanes:

Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (ab27551) at 2 µg/mL

Lane 1:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)

Lanes 2 - 3:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (reducing conditions)

Lane 4:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)

Predicted band size: 48 kDa

Observed band size: 46 kDa

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

DC-10

亚型

IgG1

偶联物

Biotin

激发波长/发射波长

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

特异性

ab27551 recognises cytokeratin peptide 18 (45 kDa) in human tissue.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2-6 µg/mL", "FlowCytIntra-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯度

IgG fraction

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 374:181-6 PubMed18621021

2008

Quantitative multiplexed quantum dot immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

E Sweeney,T H Ward,N Gray,C Womack,G Jayson,A Hughes,C Dive,R Byers

View all publications

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生物素Anti-Cytokeratin 18抗体[DC-10]

Biotin Anti-Cytokeratin 18 antibody [DC-10]

Flow Cytometry (Intracellular) - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

Western blot - Biotin Anti-Cytokeratin 18 antibody [DC-10] (AB27551)

关键信息

宿主种属

克隆

克隆号

亚型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

免疫原

特异性