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生物素Anti-CD8抗体[MEM-31] (ab28090)

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Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)
  • Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)

Key features and details

  • Biotin Mouse monoclonal [MEM-31] to CD8
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG2a

概述

  • 产品名称

    生物素Anti-CD8抗体[MEM-31]
    参阅全部 CD8 一抗

  • 描述

    生物素小鼠单克隆抗体[MEM-31] to CD8

  • 宿主

    Mouse

  • 偶联物

    Biotin

  • 经测试应用

    适用于: Flow Cytmore details
    不适用于: WB

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD8. Crude thymus membrane fraction

  • 表位

    ab28090 recognizes a conformationally dependent epitope of CD8 antigen (does not react with formaldehyde-fixed cells)

  • 常规说明

    The purified antibody is conjugated with Biotin-LC-NHS.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab28090于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
说明
Flow Cyt
Use 1µg for 106 cells.

ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
应用说明
Is unsuitable for WB.

靶标

  • 功能

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

  • 疾病相关

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻译后修饰

    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 形式

      CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.

    • 别名

      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    图片

    • Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)
      Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)

      Flow cytometry analysis (surface staining) of human peripheral blood using ab28090.

    • Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)
      Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)
      Overlay histogram showing peripheral blood lymphocytes stained with ab28090 (red line). The cells were incubated with the antibody (ab28090, 1µg/1x106 cells) followed by Streptavidin-FITC conjugated protein for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

    文献 (1)

    发表研究结果有使用 ab28090？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab28090 被引用在 1 文献中.

    • Horejsí V  et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). PubMed: 2422063

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