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AB25176

生物素Anti-CD8 alpha抗体[53-6.7]

Biotin Anti-CD8 alpha antibody [53-6.7]

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Rat Monoclonal CD8 alpha antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Tissue preparation containing Cd8a protein.

查看别名

CD8a, Lyt-2, Lyt2, Cd8a, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2

1 Images
Flow Cytometry - Biotin Anti-CD8 alpha antibody [53-6.7] (AB25176)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD8 alpha antibody [53-6.7] (AB25176)

Flow cytometry analysis showing C57Bl/6 splenocytes stained with ab25176 (solid line). Isotype control antibody was Rat Ig2a Biotin (dashed line).

不同偶联物与剂型 (8)

  • Unconjugated

    Anti-CD8 alpha antibody [53-6.7]

  • 578 PE

    PE Anti-CD8 alpha antibody [53-6.7]

  • Cy5®

    Cy5 ® Anti-CD8 alpha antibody [53-6.7]

  • 695 APC/Cy5.5®

    APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD8 alpha antibody [53-6.7]

  • 519 FITC

    FITC Anti-CD8 alpha antibody [53-6.7]

  • 578 PE

    PE Anti-CD8 alpha antibody [53-6.7]

  • 660 APC

    APC Anti-CD8 alpha antibody [53-6.7]

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

53-6.7

亚型

IgG2a

轻链类型

kappa

偶联物

Biotin

激发波长/发射波长
不含载体蛋白

No

反应种属

Mouse

应用

Flow Cyt

applications

免疫原

Tissue preparation containing Cd8a protein. The exact immunogen used to generate this antibody is proprietary information.

P01731

特异性

ab25176 recognises a chain of the murine CD8 heterodimer, Mr 32-34 kDa.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/biotin-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18445'>ab18445</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

In vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Avoid freeze / thaw cycle|Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule : peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
See full target information Cd8a
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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