重组生物素Anti-CD68抗体[KP1] (ab317601)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD68 antibody [KP1] (ab317601)](https://www.abcam.cn/ps/products/317/ab317601/Images/ab317601-2-Anti-CD68-antibody-KP1-Biotin-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD68 antibody [KP1] (ab317601)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Mouse monoclonal [KP1] to CD68
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
Related conjugates and formulations
概述
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产品名称
生物素Anti-CD68抗体[KP1]
参阅全部 CD68 一抗 -
描述
生物素小鼠单克隆抗体[KP1] to CD68 -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human tonsil.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
KP1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-CD68 antibody [KP1] (ab222914)
- Anti-CD68 antibody [KP1] - BSA and Azide free (ab233172)
- Alexa Fluor® 555 Anti-CD68 antibody [KP1] (ab279323)
- Alexa Fluor® 594 Anti-CD68 antibody [KP1] (ab282650)
- Alexa Fluor® 647 Anti-CD68 antibody [KP1] (ab314474)
- Anti-CD68 antibody [KP1], prediluted (ab845)
- Anti-CD68 antibody [KP1] (ab955)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317601于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
组织特异性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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别名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-CD68 antibody [KP1] (ab317601)
IHC image of CD68 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*.
Performed on a Leica BONDTM system. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab317601 at 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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![Biotin Anti-CD68 antibody [KP1] (ab317601)](https://www.abcam.cn/ps/products/317/ab317601/Images/ab317601-1-Benefits-of-Recombinant-antibodies.jpg)
Biotin Anti-CD68 antibody [KP1] (ab317601)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317601 尚未被引用在任何文献中。