AB134331

Biotin Anti-CD63 抗体 [MEM-259]

Name: Biotin Anti-CD63抗体[MEM-259] (ab134331)| Abcam中文官网
Brand: Abcam Limited
SKU: AB134331
Price: 5028 CNY
Availability: InStock
Rating: 3 (1 reviews)

Biotin Anti-CD63 antibody [MEM-259]

(1 Review)

(8 Publications)

Mouse Monoclonal CD63 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing CD63 protein.

查看别名

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

3 Images

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained usingab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC).

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained usingab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC) and anti-human CD203c PE antibody (20 μL reagent / 100 μL of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD63 antibody [MEM-259] (AB134331)

Flow Cytometry analysis of (surface staining) of IgE stimulated human peripheral whole blood using ab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC). CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed).

不同偶联物与剂型 (5)

Unconjugated

Anti-CD63 antibody [MEM-259]
578 PE

PE Anti-CD63 antibody [MEM-259]
667 PE/Cy5®

PE/Cy5® Anti-CD63 antibody [MEM-259]
675 PerCP

PerCP Anti-CD63 antibody [MEM-259]
519 FITC

FITC Anti-CD63 antibody [MEM-259]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-259

亚型

IgG1

偶联物

Biotin

激发波长/发射波长

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Cell preparation containing CD63 protein. The exact immunogen used to generate this antibody is proprietary information.

P08962

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

style

left-column

产品详情

The antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Size-exclusion chromatography

存储溶液

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.

Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

The protein expressed by gene CD63 functions as a cell surface receptor for TIMP1 and is involved in the activation of various cellular signaling cascades. It plays a role in the activation of ITGB1 and integrin signaling, which leads to the activation of AKT, FAK/PTK2, and MAP kinases, promoting cell survival, actin cytoskeleton reorganization, and cell adhesion, spreading, and migration through the activation of AKT and FAK/PTK2. Additionally, it participates in VEGFA signaling by regulating the internalization of KDR/VEGFR2. CD63 is involved in intracellular vesicular transport processes and is essential for the normal trafficking of the PMEL luminal domain, crucial for melanocyte development and maturation. It also contributes to leukocyte adhesion onto endothelial cells by regulating SELP trafficking. CD63 may play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in response to other stimuli. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD63

style

left-column

文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:17707 PubMed37853206

2023

Use of transcranial low-intensity focused ultrasound for targeted delivery of stem cell-derived exosomes to the brain.

Applications

Unspecified application

Species

Unspecified reactive species

J Haroon,K Aboody,L Flores,M McDonald,K Mahdavi,M Zielinski,K Jordan,E Rindner,J Surya,V Venkatraman,V Go-Stevens,G Ngai,J Lara,C Hyde,S Schafer,M Schafer,A Bystritsky,I Nardi,T Kuhn,D Ross,S Jordan

Journal of nanobiotechnology 20:530 PubMed36514065

2022

Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Eleonora Temperini,Flavio Di Giacinto,Sabrina Romanò,Riccardo Di Santo,Alberto Augello,Raffaella Polito,Leonetta Baldassarre,Valeria Giliberti,Massimiliano Papi,Umberto Basile,Benedetta Niccolini,Ewa K Krasnowska,Annalucia Serafino,Marco De Spirito,Alessandra Di Gaspare,Michele Ortolani,Gabriele Ciasca

Cell & bioscience 11:37 PubMed33568197

2021

Comparative evaluation of methods for isolating small extracellular vesicles derived from pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Min Wang,Yong-Jiang Li,Jun-Yong Wu,Jia-Xin Cai,Jing Wen,Da-Xiong Xiang,Xiong-Bin Hu,Wen-Qun Li

ACS sensors 5:764-771 PubMed32134252

2020

Enabling Sensitive Phenotypic Profiling of Cancer-Derived Small Extracellular Vesicles Using Surface-Enhanced Raman Spectroscopy Nanotags.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Lianmei Jiang,Russell J Diefenbach,Douglas H Campbell,Bradley J Walsh,Nicolle H Packer,Yuling Wang

The Journal of clinical investigation 130:1185-1198 PubMed31770109

2019

CD73 immune checkpoint defines regulatory NK cells within the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Yong Neo,Ying Yang,Julien Record,Ran Ma,Xinsong Chen,Ziqing Chen,Nicholas P Tobin,Emily Blake,Christina Seitz,Ron Thomas,Arnika Kathleen Wagner,John Andersson,Jana de Boniface,Jonas Bergh,Shannon Murray,Evren Alici,Richard Childs,Martin Johansson,Lisa S Westerberg,Felix Haglund,Johan Hartman,Andreas Lundqvist

The Analyst 144:5856-5865 PubMed31482867

2019

Exosome-specific tumor diagnosis via biomedical analysis of exosome-containing microRNA biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijin Fan,Jun Yu,Jingyan Lin,Ying Liu,Yuhui Liao

Nanoscale 10:20289-20295 PubMed30371719

2018

A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Huang,Dian-Bing Wang,Netrapal Singh,Fang Yang,Ning Gu,Xian-En Zhang

Neurobiology of disease 106:222-234 PubMed28610892

2017

Novel human neuronal tau model exhibiting neurofibrillary tangles and transcellular propagation.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Reilly,Charisse N Winston,Kelsey R Baron,Margarita Trejo,Edward M Rockenstein,Johnny C Akers,Najla Kfoury,Marc Diamond,Eliezer Masliah,Robert A Rissman,Shauna H Yuan

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Biotin Anti-CD63 抗体 [MEM-259]