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AB28069

生物素Anti-CD4抗体[MEM-241]

Biotin Anti-CD4 antibody [MEM-241]

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(2 Publications)

Mouse Monoclonal CD4 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD4.

查看别名

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

2 Images
Flow Cytometry - Biotin Anti-CD4 antibody [MEM-241] (AB28069)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD4 antibody [MEM-241] (AB28069)

Separation of human CD4 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab28069 (concentration in sample 2 µg/ml) and secondary stained using anti-biotin (1D4-C5) Alexa Fluor® 647, concentration in sample 0.19 µg/ml).

Flow Cytometry - Biotin Anti-CD4 antibody [MEM-241] (AB28069)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-CD4 antibody [MEM-241] (AB28069)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28069 (concentration in sample 2 µg/ml) and secondary stained using anti-biotin (1D4-C5) Alexa Fluor® 647 (concentration in sample 0.19 µg/ml).

不同偶联物与剂型 (7)

  • 660 APC

    APC Anti-CD4 antibody [MEM-241]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD4 antibody [MEM-241]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD4 antibody [MEM-241]

  • 519 FITC

    FITC Anti-CD4 antibody [MEM-241]

  • 578 PE

    PE Anti-CD4 antibody [MEM-241]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD4 antibody [MEM-241]

  • 675 PerCP

    PerCP Anti-CD4 antibody [MEM-241]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-241

亚型

IgG1

轻链类型

kappa

偶联物

Biotin

激发波长/发射波长
不含载体蛋白

No

反应种属

Human

应用

Flow Cyt

applications

免疫原

Recombinant Fragment Protein within Human CD4. The exact immunogen used to generate this antibody is proprietary information.

P01730

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/biotin-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab18434'>ab18434</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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产品详情

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
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性能和储存信息

形式
Liquid
纯化工艺
Size-exclusion chromatography
纯化说明
Purity >95% by SDS-PAGE.
存储溶液
pH: 7.4 Preservative: 0.097% Sodium azide
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Biosensors 12: PubMed35049640

2021

Development of a Microfluidic Device for CD4 T Cell Isolation and Automated Enumeration from Whole Blood.

Applications

Unspecified application

Species

Unspecified reactive species

Robert D Fennell,Mazhar Sher,Waseem Asghar

ACS central science 2:75-9 PubMed27163031

2016

Quantitatively Resolving Ligand-Receptor Bonds on Cell Surfaces Using Force-Induced Remnant Magnetization Spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ting Chen,Andrew C Jamison,T Randall Lee,Shoujun Xu
View all publications
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搭配使用产品

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  • 0
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