AB21889

生物素Anti-CD14抗体[MEM-18]

Name: Biotin Anti-CD14抗体[MEM-18] (ab21889)| Abcam中文官网
Brand: Abcam Limited
SKU: AB21889
Price: 4885 CNY
Availability: InStock

Biotin Anti-CD14 antibody [MEM-18]

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(1 Publication)

Mouse Monoclonal CD14 antibody - conjugated to Biotin. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.

查看别名

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

2 Images

Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (AB21889)

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (AB21889)

Separation of human monocytes (red) from lymphocytes (black) in flow cytometry analysis (surface staining) of human peripheral whole blood using ab21889 at 6 μg/ml, Streptavidin.

Flow Cyt

Unknown

Flow Cytometry - Biotin Anti-CD14 antibody [MEM-18] (AB21889)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab21889 at 6 μg/ml.

不同偶联物与剂型 (1)

Carrier free

Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

MEM-18

亚型

IgG1

偶联物

Biotin

激发波长/发射波长

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human CD14.

P08571

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-4 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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产品详情

Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.

Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).

See full target information CD14

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Plant physiology and biochemistry : PPB 140:78-87 PubMed31085449

2019

Heat stress reveals high molecular mass proteasomes in Arabidopsis thaliana suspension cells cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Aristizábal,Viridiana Rivas,Gladys I Cassab,Fernando Lledías

View all publications

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生物素Anti-CD14抗体[MEM-18]