生物素Anti-beta Galactosidase抗体(ab6645)
Key features and details
- Biotin Rabbit polyclonal to beta Galactosidase
- Suitable for: Dot blot, ELISA, WB, IHC-P
- Reacts with: Escherichia coli
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-beta Galactosidase抗体
参阅全部 beta Galactosidase 一抗 -
描述
生物素兔多克隆抗体to beta Galactosidase -
宿主
Rabbit -
偶联物
Biotin -
经测试应用
适用于: Dot blot, ELISA, WB, IHC-Pmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Full length native protein (purified) corresponding to Escherichia coli beta Galactosidase.
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常规说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
Anti-BETA GALACTOSIDASE Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
1/2000 - 1/10000.
|
|
| IHC-P |
1/1000 - 1/5000.
|
| 说明 |
|---|
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
1/10000 - 1/50000. |
|
WB
1/2000 - 1/10000. |
|
IHC-P
1/1000 - 1/5000. |
靶标
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相关性
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
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别名
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
图片
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Western blot - Biotin Anti-beta Galactosidase antibody (ab6645)Biotin Anti-beta Galactosidase antibody (ab6645) at 1/1000 dilution + Beta Galactosidase at 0.05 µg with Blocking buffer
Secondary
HRP streptavidin conjugate at 1/40000 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab6645 被引用在 4 文献中.
- Matsumoto K et al. In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay). Sci Rep 7:12136 (2017). PubMed: 28939819
- Herrera JJ et al. Sustained expression of vascular endothelial growth factor and angiopoietin-1 improves blood-spinal cord barrier integrity and functional recovery after spinal cord injury. J Neurotrauma 27:2067-76 (2010). IHC-Fr ; Rat . PubMed: 20799882
- Siembab VC et al. Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells. J Comp Neurol 518:4675-701 (2010). PubMed: 20963823
- Glaser T et al. Generation of purified oligodendrocyte progenitors from embryonic stem cells. FASEB J 19:112-4 (2005). PubMed: 15486057