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AB21899

Biotin Anti-beta 2 Microglobulin 抗体 [B2M-01]

Biotin Anti-beta 2 Microglobulin antibody [B2M-01]

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(3 Publications)

Mouse Monoclonal beta 2 Microglobulin antibody - conjugated to Biotin. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human B2M.

查看别名

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

2 Images
Flow Cytometry - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (AB21899)
  • Flow Cyt

Supplier Data

Flow Cytometry - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (AB21899)

Flow cytometry analysis using anti-beta2 microglobulin (B2M-01) biotin for surface staining of beta2-microglobulin in human peripheral blood

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (AB21899)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (AB21899)

IHC image of beta 2 Microglobulin staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21899, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

不同偶联物与剂型 (3)

  • Unconjugated

    Anti-beta 2 Microglobulin antibody [B2M-01]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-beta 2 Microglobulin antibody [B2M-01]

  • 578 PE

    PE Anti-beta 2 Microglobulin antibody [B2M-01]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

B2M-01

亚型

IgG2a

偶联物

Biotin

激发波长/发射波长
不含载体蛋白

No

反应种属

Human

应用

Flow Cyt, IHC-P

applications

免疫原

Native Full Length Protein corresponding to Human B2M.

P61769

特异性

This antibody reacts with beta 2 Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 Microglobulin.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Chicken": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Dog": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rabbit": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.
Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).
See full target information B2M
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36125261

2022

Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation.

Applications

Unspecified application

Species

Unspecified reactive species

Derek M Britain,Jason P Town,Orion David Weiner

Methods (San Diego, Calif.) 59:328-35 PubMed23270815

2012

A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Huaying Zhao,Inna I Gorshkova,Gregory L Fu,Peter Schuck

Clinical biochemistry 43:367-72 PubMed20006594

2009

FcRn binding properties of an abnormal truncated analbuminemic albumin variant.

Applications

ELISA

Species

Human

Jan Terje Andersen,Muluneh Bekele Daba,Inger Sandlie
View all publications
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