生物素Anti-bacterial Alkaline Phosphatase抗体(ab7321)
Key features and details
- Biotin Rabbit polyclonal to bacterial Alkaline Phosphatase
- Suitable for: WB, ELISA
- Reacts with: Escherichia coli
- Conjugation: Biotin
- Isotype: IgG
概述
-
产品名称
生物素Anti-bacterial Alkaline Phosphatase抗体 -
描述
生物素兔多克隆抗体to bacterial Alkaline Phosphatase -
宿主
Rabbit -
偶联物
Biotin -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Full length native protein (purified) corresponding to Escherichia coli bacterial Alkaline Phosphatase.
-
常规说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Lyophilized:Reconstitute product with 100ul 50:50 water:glycerol. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| ELISA |
| 说明 |
|---|
ELISA (1:4,000 to 1:20,000)
Immuno microscopy
Immunohistochemistry
as well as other peroxidase-antibody based enzymatic assays.
靶标
-
相关性
Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies. -
细胞定位
Periplasm -
数据库链接
- Entrez Gene: 945041 Escherichia coli
- SwissProt: P00634 Escherichia coli
-
别名
- Alkaline phosphatase antibody
- APase antibody
- BAP antibody
- PhoA antibody
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab7321 被引用在 1 文献中.
- Alcaine SD et al. Bioengineering bacteriophages to enhance the sensitivity of phage amplification-based paper fluidic detection of bacteria. Biosens Bioelectron 82:14-9 (2016). PubMed: 27031186