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AB7321

生物素Anti-bacterial Alkaline Phosphatase抗体

Biotin Anti-bacterial Alkaline Phosphatase antibody

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(2 Publications)

Rabbit Polyclonal PPB antibody - conjugated to Biotin. Suitable for ELISA, WB and reacts with Escherichia coli samples. Cited in 2 publications.

查看别名

b0383, JW0374, phoA, Alkaline phosphatase, APase

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

偶联物

Biotin

激发波长/发射波长
不含载体蛋白

No

反应种属

Escherichia coli

应用

ELISA, WB

applications

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/4000 - 1/20000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

产品详情

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

性能和储存信息

形式
Liquid
纯度
IgG fraction
纯化工艺
Ion exchange chromatography
纯化说明
Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
存储溶液
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Bacterial Alkaline Phosphatase often referred to as E. coli Alkaline Phosphatase is an enzyme that removes phosphate groups from molecules such as nucleotides proteins and alkaloids. It possesses a mass of approximately 47 kDa functioning optimally at a pH above 8.0. This enzyme is prominently expressed in the periplasmic space of Escherichia coli functioning as a homodimer where each subunit contributes to the active site essential for its catalytic action.
Biological function summary

Bacterial alkaline phosphatase participates in dephosphorylation reactions facilitating the release of inorganic phosphate. Its enzymatic activities are important for bacterial growth and metabolism enabling bacteria to utilize extracellular organic phosphates. While not explicitly part of a complex the enzyme interacts with various substrates reflecting its role in dynamic regulatory processes within the bacterial cell. It plays a particularly important part during phosphate starvation as it allows bacteria to scavenge phosphate from different organic groups.

Pathways

Bacterial alkaline phosphatase integrates into the phosphorus assimilation and recycling pathways essential for cellular function and survival. In the phosphate acquisition pathway it works in conjunction with proteins like PhoR and PhoB that regulate the expression of phosphate-responsive genes. Its action ensures the maintenance of phosphate homeostasis which in turn influences broader bacterial physiological processes. Direct interaction with PhoA and PhoU aids the regulation of phosphate uptake in bacteria through the two-component system.

Bacterial alkaline phosphatase plays roles in understanding certain bacterial infections and their adaptations. It specifically associates with bacterial pathogenicity and antibiotic resistance which present significant challenges in clinical settings. The enzyme's ability to thrive in phosphate-limited environments links it to virulence in pathogenic strains like E. coli and its relationship with other virulence factors might disrupt host cell processes aiding disease progression. Understanding its interaction with proteins associated with bacterial invasion and survival can provide insights into treatment of infections caused by resistant bacterial strains.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Biosensors & bioelectronics 82:14-9 PubMed27031186

2016

Bioengineering bacteriophages to enhance the sensitivity of phage amplification-based paper fluidic detection of bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

S D Alcaine,K Law,S Ho,A J Kinchla,D A Sela,S R Nugen

FEMS microbiology letters 275:71-9 PubMed17662058

2007

Membrane topology of the GltS Na+/glutamate permease of Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Attila Szvetnik,József Gál,Miklós Kálmán
View all publications

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