Anti-Biotin抗体(ab53494)
Key features and details
- Rabbit polyclonal to Biotin
- Suitable for: WB, Competitive ELISA, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-Biotin抗体
参阅全部 Biotin 一抗 -
描述
兔多克隆抗体to生物素 -
宿主
Rabbit -
特异性
Reacts with free biotin and biotinylated antibody and proteins -
经测试应用
适用于: WB, Competitive ELISA, IP, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.00
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity purified with d-Biotin-agarose beads -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53494于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
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| Competitive ELISA |
Use at an assay dependent dilution. (sensitivity: 0.01 ng/mL).
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| IP | (1) |
Use at an assay dependent dilution.
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| ICC/IF |
Use at an assay dependent dilution.
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| 说明 |
|---|
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WB
Use at an assay dependent dilution. Predicted molecular weight: 1 kDa. |
|
Competitive ELISA
Use at an assay dependent dilution. (sensitivity: 0.01 ng/mL). |
|
IP
Use at an assay dependent dilution. |
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ICC/IF
Use at an assay dependent dilution. |
靶标
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相关性
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
别名
- Vitamin B4 antibody
图片
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Immunoprecipitation - Anti-Biotin antibody (ab53494)Image courtesy of an anonymous AbReview
RWP1 cells were lysed with Polysomal Lysis
Buffer and diluted in binding buffer (20 mM Hepes
pH 7.6, 150 mM KCl, 3 mM MgCl2, 10% glycerol, 3 mg/ml BSA, 0.2 Triton X-100, 20 µg/ml poly dI-dC, 1 mM DDT, plus protease and RNase inhibitors). Pre-clearing was performed by incubating with protein G-agarose blocked with salmon sperm (1 mg/mL) and mouse IgG (10 µg/mL). After pre-clearing, samples (500 µg) were incubated 4 hours in binding buffer with the DNA-biotinylated probes (2 µg) and antibody against biotin (20 µg/mL) in a final volume of 1 mL. Samples were pulled-down with protein G-agarose, washed with binding buffer supplemented with 0.1%
Tween-20 and analyzed by Western Blot for Suz12.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (56)
ab53494 被引用在 56 文献中.
- Erdem JS et al. High aspect ratio nanomaterial-induced macrophage polarization is mediated by changes in miRNA levels. Front Immunol 14:1111123 (2023). PubMed: 36776851
- Pavluch V et al. Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells. Sci Rep 13:5788 (2023). PubMed: 37031254
- Meurant S et al. Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37298552
- Mayoral J et al. Dense Granule Protein GRA64 Interacts with Host Cell ESCRT Proteins during Toxoplasma gondii Infection. mBio 13:e0144222 (2022). PubMed: 35730903
- Dong X et al. Keratinocyte-derived defensins activate neutrophil-specific receptors Mrgpra2a/b to prevent skin dysbiosis and bacterial infection. Immunity 55:1645-1662.e7 (2022). PubMed: 35882236