Anti-Biotin抗体(ab1227)
Key features and details
- Rabbit polyclonal to Biotin
- Suitable for: ICC/IF, WB, IHC, ELISA, IP
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-Biotin抗体
参阅全部 Biotin 一抗 -
描述
兔多克隆抗体to生物素 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC, ELISA, IPmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin. Rabbits were immunized with KLH-Biotin. After multiple immunizations in Freund's adjuvant the rabbits were bled and serum collected.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.1% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
1/200 - 1/2000.
|
| WB | (3) |
1/1000 - 1/30000.
|
| IHC |
1/200 - 1/2000.
|
|
| ELISA |
1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
|
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| IP | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
1/200 - 1/2000. |
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WB
1/1000 - 1/30000. |
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IHC
1/200 - 1/2000. |
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ELISA
1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000. |
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IP
Use at an assay dependent concentration. |
靶标
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相关性
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
别名
- Vitamin B4 antibody
图片
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Western blot - Anti-Biotin antibody (ab1227)This image is courtesy of an Abreview submitted by Mr John AndersenAnti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Secondary
Streptavidin-HRP
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab1227 被引用在 18 文献中.
- Yu J et al. Genome-wide association studies identify OsWRKY53 as a key regulator of salt tolerance in rice. Nat Commun 14:3550 (2023). PubMed: 37321989
- Cui C et al. Inhibitory Peptide of Soluble Guanylyl Cyclase/Trx1 Interface Blunts the Dual Redox Signaling Functions of the Complex. Antioxidants (Basel) 12:N/A (2023). PubMed: 37107281
- Donnio LM et al. XAB2 dynamics during DNA damage-dependent transcription inhibition. Elife 11:N/A (2022). PubMed: 35880862
- Qi Q et al. A novel posttranslational modification of histone, H3 S-sulfhydration, is down-regulated in asthenozoospermic sperm. J Assist Reprod Genet 38:3175-3193 (2021). PubMed: 34664184
- Chen G et al. bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway. Redox Biol 41:101904 (2021). PubMed: 33706169