生物素Anti-5-methylcytosine (5-mC)抗体[5MC-CD] - Azide free (ab179914)
![Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)](https://www.abcam.cn/ps/products/179/ab179914/Images/ab179914-209594-anti-5-methylcytosine-5-mc-antibody-5mc-cd-azide-free-biotin-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)")
Key features and details
- Biotin Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) - Azide free
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgM
Related conjugates and formulations
概述
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产品名称
生物素Anti-5-methylcytosine (5-mC)抗体[5MC-CD] - Azide free
参阅全部 5-methylcytosine (5-mC) 一抗 -
描述
生物素小鼠单克隆抗体[5MC-CD] to 5-methylcytosine (5-mC) - Azide free -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).
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阳性对照
- Chlamydomonas me-1 and mouse embryonic stem cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Filter-sterilized. -
Concentration information loading... -
纯度
Purified IgM -
克隆
单克隆 -
克隆编号
5MC-CD -
同种型
IgM -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179914于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
|
| 说明 |
|---|
|
ICC/IF
1/50 - 1/100. |
靶标
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形式
The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application. -
别名
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)
Immunofluorescence analysis of Clamydomonas me-1 cells, labeling 5-Methyl Cytosine using ab179914 at a 1/50 dilution, followed by FITC-conjugated secondary antibody (green). DAPI was used to stain DNA (magenta). Upper panels (a, b and c) correspond to vegetative cells and lower panels (d, e and f) to gametes.
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![Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)](https://www.abcam.cn/ps/products/179/ab179914/Images/ab179914-209595-anti-5-methylcytosine-5-mc-antibody-5mc-cd-azide-free-biotin-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)Immunofluorescence analysis of mouse embryonic cells, labeling 5-Methyl Cytosine using ab179914 at a 1/50 dilution. DAPI was used to stain DNA (magenta).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab179914 被引用在 1 文献中.
- Liang Y et al. Magnetic Immunosensor Coupled to Enzymatic Signal for Determination of Genomic DNA Methylation. Biosensors (Basel) 12:N/A (2022). PubMed: 35323432