Anti-BIN1抗体(ab27796)
Key features and details
- Rabbit polyclonal to BIN1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BIN1抗体
参阅全部 BIN1 一抗 -
描述
兔多克隆抗体to BIN1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Mouse BIN1. Corresponding to region surrounding amino acid 578
Database link: O08539-1 -
阳性对照
- Jurkat cells
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3672 Bin1 Antibody. 3672-100 is the same size as the 100 µg size of ab27796.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27796于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. -
组织特异性
Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle. -
疾病相关
Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. -
序列相似性
Contains 1 BAR domain.
Contains 1 SH3 domain. -
翻译后修饰
Phosphorylated by protein kinase C. -
细胞定位
Cytoplasm and Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 274 Human
- Omim: 601248 Human
- SwissProt: O00499 Human
- Unigene: 193163 Human
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别名
- AMPH 2 antibody
- AMPH2 antibody
- Amphiphysin 2 antibody
see all
图片
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Western blot - Anti-BIN1 antibody (ab27796)All lanes : Anti-BIN1 antibody (ab27796) at 2 µg/ml
Lane 1 : Jurkat whole cell lysate (ab7899)
Lane 2 : Jurkat cells treated with camptothecin for 6 hours
Predicted band size: 65 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa (possible cleavage fragment)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab27796 被引用在 2 文献中.
- Lambert E et al. The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects. Acta Neuropathol Commun 10:4 (2022). PubMed: 34998435
- Chapuis J et al. Increased expression of BIN1 mediates Alzheimer genetic risk by modulating tau pathology. Mol Psychiatry N/A:N/A (2013). WB ; Human . PubMed: 23399914