AB17935

Anti-Bim (phospho S65)抗体

Name: Bim(磷酸化S65)抗体 (ab17935)| Abcam中文官网
Brand: Abcam Limited
SKU: AB17935
Price: 4606 CNY
Availability: InStock

Anti-Bim (phospho S65) antibody

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(7 Publications)

Rabbit Polyclonal Bim phospho S65 antibody. Suitable for WB and reacts with Synthetic peptide - Rat samples. Cited in 7 publications.

查看别名

Bim, Bod, Bcl2l11, Bcl-2-like protein 11, Bcl2-L-11, Bcl-2-related ovarian death protein, Bcl2-interacting mediator of cell death

1 Images

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Western blot - Anti-Bim (phospho S65) antibody (AB17935)

Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2, 5) or with
mutant S65A Bim and MEKK1 (3, 6), were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or ab17935 (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with a goat F(ab')2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.

The data show that the signal is detected only in lysates co-expressing rat WT Bim and active MEKK1. This signal is abolished in cells expressing mutant rat Bim S65A and MEKK1, verifying that the signal is site-phosphospecific.

Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2

All lanes:

Western blot - Anti-Bim (phospho S65) antibody (ab17935)

Predicted band size: 22 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synthetic peptide - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).

存储溶液

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Bim also known as Bcl-2-interacting mediator of cell death is an important pro-apoptotic protein within the Bcl-2 family. It has a molecular weight of approximately 23 kDa. Bim is expressed in various tissues including the immune system nervous system and lymphoid tissues. It exists in multiple isoforms such as BimEL BimL and BimS due to alternative splicing. Bim's interaction with cellular membranes allows it to regulate apoptotic processes through mitochondrial pathways effectively.

Biological function summary

Bim regulates apoptosis by binding to pro-survival proteins like Bcl-2 and Bcl-xL releasing pro-apoptotic factors from mitochondria and activating caspases. Bim acts as part of the apoptosome complex and influences cell death regulation significantly. By promoting cytochrome c release from mitochondria Bim initiates a cascade of events leading to cell apoptosis. This regulation is vital in maintaining the balance between cell survival and death necessary for normal development and tissue homeostasis.

Pathways

Bim plays a critical role in the intrinsic apoptotic pathway. This pathway involves mitochondrial outer membrane permeabilization where Bim interacts with several Bcl-2 family proteins such as Bax and Bak to induce apoptosis. The modulation of Bim expression and activity is influenced by growth factor signaling pathways such as the PI3K-Akt pathway which affects Bim phosphorylation leading to its inactivation and subsequent degradation. Therefore Bim acts as an important node linking survival signals and apoptotic machinery.

Bim dysregulation has been implicated in conditions like cancer and autoimmune diseases. In certain cancers reduced Bim expression can result in unchecked cell proliferation and resistance to apoptosis. Conversely in autoimmune disorders overactivity of Bim may lead to excessive immune cell apoptosis contributing to disease pathogenesis. Bim's interactions with proteins like Bcl-2 and Bcl-xL are significant in cancer therapy as targeting these interactions can help overcome resistance to apoptosis in cancer cells improving treatment outcomes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

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靶点信息

Induces apoptosis and anoikis.

See full target information Bcl2l11 pS65

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1491546 PubMed39584135

2024

Huyang Yangkun formula regulates the mitochondria pathway of ovarian granulosa cell apoptosis through FTO/m6A-P53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Lingdi Wang,Jian Liu,Guangning Nie,Hongyan Yang

Evidence-based complementary and alternative medicine : eCAM 2021:6614302 PubMed34035823

2021

Danggui Buxue Tang Rescues Folliculogenesis and Ovarian Cell Apoptosis in Rats with Premature Ovarian Insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdi Wang,Jian Liu,Guangning Nie,Yang Li,Hongyan Yang

General physiology and biophysics 39:145-155 PubMed32329442

2020

Inhibition of HES-1 might play a protective role in endothelial cells under cholesterol stimulation via PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Shi,Ya Su,Chuanli Liu,Jinglong Tao,Hao Fu,Chengzhi Lu

Oncology reports 42:1183-1193 PubMed31233204

2019

Synergistic antitumor effect of BRMS1 and sorafenib via inhibition of the PI3K/AKT/mTOR/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyun Jin,Yonggang Guo,Yang Liu,Zhenguo Wang,Xiaosa Yang,Shengkai Sun

PloS one 10:e0132998 PubMed26171786

2015

Involvement of the JNK/FOXO3a/Bim Pathway in Neuronal Apoptosis after Hypoxic-Ischemic Brain Damage in Neonatal Rats.

Applications

Species

Rat

Deyuan Li,Xihong Li,Jinlin Wu,Jinhui Li,Li Zhang,Tao Xiong,Jun Tang,Yi Qu,Dezhi Mu

Proceedings of the National Academy of Sciences of the United States of America 110:E4894-903 PubMed24282297

2013

Tuberous sclerosis 1 (Tsc1)-dependent metabolic checkpoint controls development of dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Wang,Gonghua Huang,Hu Zeng,Kai Yang,Richard F Lamb,Hongbo Chi

Proceedings of the National Academy of Sciences of the United States of America 109:E343-52 PubMed22308391

2012

Transforming growth factor beta-activated kinase 1 (TAK1)-dependent checkpoint in the survival of dendritic cells promotes immune homeostasis and function.

Applications

Species

Mouse

Yanyan Wang,Gonghua Huang,Peter Vogel,Geoffrey Neale,Boris Reizis,Hongbo Chi

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