Anti-Bik抗体(ab62494)
Key features and details
- Rabbit polyclonal to Bik
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Bik抗体
参阅全部 Bik 一抗 -
描述
兔多克隆抗体to Bik -
宿主
Rabbit -
经测试应用
适用于: WB, ICC, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Bik (N terminal). 15 amino acid synthetic peptide from near the amino terminus
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阳性对照
- Jurkat whole cell lysate (ab7899) and Jurkat cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62494于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).
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| ICC |
Use a concentration of 1 - 20 µg/ml.
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| ICC/IF |
Use a concentration of 1 - 20 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa). |
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ICC
Use a concentration of 1 - 20 µg/ml. |
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ICC/IF
Use a concentration of 1 - 20 µg/ml. |
靶标
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功能
Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. -
结构域
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
细胞定位
Endomembrane system. Around the nuclear envelope, and in cytoplasmic membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 638 Human
- Omim: 603392 Human
- SwissProt: Q13323 Human
- Unigene: 475055 Human
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别名
- Apoptosis inducer NBK antibody
- BBC1 antibody
- Bcl-2-interacting killer antibody
see all
图片
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Western blot - Anti-Bik antibody - Aminoterminal end (ab62494)Lane 1 : Anti-Bik antibody (ab62494) at 1 µg/ml
Lane 2 : Anti-Bik antibody (ab62494) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-Bik antibody - Aminoterminal end (ab62494)Immunocytochemistry of Bik in Jurkat cells with ab62494 antibody at 1 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Bik antibody (ab62494)Immunofluorescence of Bik in Jurkat cells using ab62494 at 10 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62494 尚未被引用在任何文献中。