Anti-Bik抗体(ab52182)
Key features and details
- Rabbit polyclonal to Bik
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Bik抗体
参阅全部 Bik 一抗 -
描述
兔多克隆抗体to Bik -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Bik. Non-phosphopeptide from around the phosphorylation site of threonine 33 (G-M-T-D-S).
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阳性对照
- Extracts from A549 cells treated with DMSO, human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52182于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
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| WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 18 kDa.
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| ICC/IF | (1) |
Use a concentration of 1 - 5 µg/ml.
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Predicted molecular weight: 18 kDa. |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. -
结构域
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
细胞定位
Endomembrane system. Around the nuclear envelope, and in cytoplasmic membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 638 Human
- Omim: 603392 Human
- SwissProt: Q13323 Human
- Unigene: 475055 Human
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别名
- Apoptosis inducer NBK antibody
- BBC1 antibody
- Bcl-2-interacting killer antibody
see all
图片
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Western blot - Anti-Bik antibody (ab52182)All lanes : Anti-Bik antibody (ab52182) at 1/500 dilution
Lane 1 : extracts from A549 cells, treated
with DMSO (0.1%, 10mins)
Lane 2 : extracts from A549 cells, treated
with DMSO (0.1%, 10mins) with blocking peptide
Predicted band size: 18 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bik antibody (ab52182)Ab52182 at 1/50 dilution staining Human breast carcinoma tissue, with and without blocking peptide; paraffin embedded. -
Immunocytochemistry/ Immunofluorescence - Anti-Bik antibody (ab52182)ICC/IF image of ab52182 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52182, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-Bik antibody (ab52182)Image courtesy of an anonymous Abreview.ab52182 staining Bik in human Jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% serum for 3 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 647 (pink) used at a 1/1000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab52182 被引用在 11 文献中.
- Nakamura K et al. Combination of Everolimus and Bortezomib Inhibits the Growth and Metastasis of Bone and Soft Tissue Sarcomas via JNK/p38/ERK MAPK and AKT Pathways. Cancers (Basel) 15:N/A (2023). PubMed: 37173935
- Xia K et al. Increased FOXA1 levels induce apoptosis and inhibit proliferation in FOXA1-low expressing basal breast cancer cells. Am J Cancer Res 12:2641-2658 (2022). PubMed: 35812039
- Duan N et al. Overexpression of HIPK2 removes the transrepression of proapoptotic genes mediated by the CtBP1-p300-FOXO3a complex and increases the chemosensitivity in osteosarcoma cells. J Cancer 12:1826-1837 (2021). PubMed: 33613771
- Xu T et al. Gene Amplification-Driven Long Noncoding RNA SNHG17 Regulates Cell Proliferation and Migration in Human Non-Small-Cell Lung Cancer. Mol Ther Nucleic Acids 17:405-413 (2019). PubMed: 31310946
- Kabir S et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695