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重组RabMAb

重组Anti-Biglycan抗体[EPR2959] (ab109369)

Reviews (1) Submit a question References (2)
Flow Cytometry (Intracellular) - Anti-Biglycan antibody [EPR2959] (ab109369)
  • Western blot - Anti-Biglycan antibody [EPR2959] (ab109369)
  • Anti-Biglycan antibody [EPR2959] (ab109369)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2959] to Biglycan
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Biglycan抗体[EPR2959]
    参阅全部 Biglycan 一抗

  • 描述

    兔单克隆抗体[EPR2959] to Biglycan

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Rat cartilage, C2C12, Human skeletal muscle and Mouse muscle lysates; L6 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109369于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
应用说明
Is unsuitable for ICC/IF or IHC-P.

靶标

  • 功能

    May be involved in collagen fiber assembly.

  • 组织特异性

    Found in several connective tissues, especially in articular cartilages.

  • 序列相似性

    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.

  • 翻译后修饰

    The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • BGN antibody
    • Biglycan antibody
    • Biglycan proteoglycan antibody
    • Bone/cartilage proteoglycan I antibody
    • Dermatan sulphate proteoglycan I antibody
    • DSPG1 antibody
    • PG S1 antibody
    • PG-S1 antibody
    • PGI antibody
    • PGS1_HUMAN antibody
    • SEMDX antibody
    • SLRR1A antibody
    • Small leucine rich protein 1A antibody
    see all

图片

  • Flow Cytometry (Intracellular) - Anti-Biglycan antibody [EPR2959] (ab109369)
    Flow Cytometry (Intracellular) - Anti-Biglycan antibody [EPR2959] (ab109369)

    Intracellular flow cytometric analysis of permeabilized L6 cells using ab109369 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

     

  • Western blot - Anti-Biglycan antibody [EPR2959] (ab109369)
    Western blot - Anti-Biglycan antibody [EPR2959] (ab109369)
    All lanes : Anti-Biglycan antibody [EPR2959] (ab109369) at 1/1000 dilution

    Lane 1 : Rat cartilage lysate
    Lane 2 : L6 lysate
    Lane 3 : C2C12 lysate
    Lane 4 : Human skeletal muscle lysate
    Lane 5 : Mouse muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 42 kDa

  • Anti-Biglycan antibody [EPR2959] (ab109369)
    Anti-Biglycan antibody [EPR2959] (ab109369)

文献 (2)

发表研究结果有使用 ab109369？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109369 被引用在 2 文献中.

  • Liu S  et al. Tyrosine kinase LYN is an oncotarget in human cervical cancer: A quantitative proteomic based study. Oncotarget 7:75468-75481 (2016). WB ; Human . PubMed: 27690342
  • Tan HT  et al. Unravelling the proteome of degenerative human mitral valves. Proteomics 15:2934-44 (2015). WB ; Human . PubMed: 25914152

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Immunohistochemistry (Frozen sections) abreview for Anti-Biglycan antibody [EPR2959]

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (Skeleton)
Permeabilization
No
Specification
Skeleton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 12 2014

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