Anti-Biglycan抗体(ab58562)
Key features and details
- Goat polyclonal to Biglycan
- Suitable for: ICC
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Biglycan抗体
参阅全部 Biglycan 一抗 -
描述
山羊多克隆抗体to Biglycan -
宿主
Goat -
经测试应用
适用于: ICCmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Sheep, Horse, Dog, Xenopus laevis, Pufferfish, Zebrafish
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免疫原
Synthetic peptide within Human Biglycan aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P21810 -
阳性对照
- ICC: Human HepG2 and Mouse NIH/3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58562于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
May be involved in collagen fiber assembly. -
组织特异性
Found in several connective tissues, especially in articular cartilages. -
序列相似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
翻译后修饰
The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 403905 Dog
- Entrez Gene: 100033879 Horse
- Entrez Gene: 633 Human
- Entrez Gene: 12111 Mouse
- Entrez Gene: 25181 Rat
- Entrez Gene: 443011 Sheep
- Entrez Gene: 399351 Xenopus laevis
- Omim: 301870 Human
see all -
别名
- BGN antibody
- Biglycan antibody
- Biglycan proteoglycan antibody
see all
图片
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Immunocytochemistry - Anti-Biglycan antibody (ab58562)
Immunocytochemistry analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized HepG2 cells staining biglycan with ab58562 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2µg/ml (green). Nuclei counterstained with DAPI (blue).
Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).
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Immunocytochemistry - Anti-Biglycan antibody (ab58562)Immunocytochemistry analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized NIH3T3 cells staining biglycan with ab58562 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2µg/ml (green). Nuclei counterstained with DAPI (blue).
Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).
数据表及文件
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Datasheet download
文献 (22)
ab58562 被引用在 22 文献中.
- Yu M et al. Biglycan promotes hepatic fibrosis through activating heat shock protein 47. Liver Int 43:500-512 (2023). PubMed: 36371672
- Zeng S et al. Aberrant expression of the extracellular matrix component Biglycan regulated by Hedgehog signalling promotes colorectal cancer cell proliferation. Acta Biochim Biophys Sin (Shanghai) 54:243-251 (2022). PubMed: 35130618
- Yin Y et al. Bmi1 regulate tooth and mandible development by inhibiting p16 signal pathway. J Cell Mol Med 25:4195-4203 (2021). PubMed: 33745198
- Zhou C et al. Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease. Aging (Albany NY) 11:7257-7273 (2019). PubMed: 31509519
- Yokoi M et al. Effect of attenuation of fibroblast growth factor receptor 2b signaling on odontoblast differentiation and dentin formation. In Vitro Cell Dev Biol Anim 55:211-219 (2019). PubMed: 30756235
