重组Anti-BIG-2抗体[EPR8736] (ab131285)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8736] to BIG-2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BIG-2抗体[EPR8736]
参阅全部 BIG-2 一抗 -
描述
兔单克隆抗体[EPR8736] to BIG-2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC,IHC-Fr or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fetal brain lysate, Human cerebellum lysate. Human kidney tissue and Human brain tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8736 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 113 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis. -
组织特异性
Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex. -
疾病相关
A chromosomal aberration involving CNTN4 has been found in a boy with characteristic physical features of 3p deletion syndrome (3PDS). Translocation t(3;10)(p26;q26). 3PDS is a rare contiguous gene disorder involving the loss of the telomeric portion of the short arm of chromosome 3 and characterized by developmental delay, growth retardation, and dysmorphic features. -
序列相似性
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
细胞定位
Cell membrane. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 152330 Human
- Entrez Gene: 269784 Mouse
- Entrez Gene: 116658 Rat
- Omim: 607280 Human
- SwissProt: Q8IWV2 Human
- SwissProt: Q69Z26 Mouse
- SwissProt: Q62845 Rat
- Unigene: 298705 Human
see all -
别名
- AXCAM antibody
- Axonal associated cell adhesion molecule antibody
- BIG 2 antibody
see all
图片
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All lanes : Anti-BIG-2 antibody [EPR8736] (ab131285) at 1/1000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 113 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelled with ab131285 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab131285 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab131285 尚未被引用在任何文献中。