重组Anti-Bid抗体[EPR27444-102] (ab317809)
![Western blot - Anti-Bid antibody [EPR27444-102] (ab317809)](https://www.abcam.cn/ps/products/317/ab317809/Images/ab317809-542626-anti-bid-antibody-epr27444-102-western-blot-untreated-l-929-mouse-connective-tissue-fibroblast-whole-cell-lysate-50-ug.jpg "Western blot - Anti-Bid antibody [EPR27444-102] (ab317809)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27444-102] to Bid
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-Bid抗体[EPR27444-102]
参阅全部 Bid 一抗 -
描述
兔单克隆抗体[EPR27444-102] to Bid -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF or IHC-P -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Untreated L-929 whole cell, L-929 treated with 20ng /ml TNF alpha and 10µM BV-6 for 10h whole cell, C2C12 whole cell, Mouse spleen tissue and Mouse thymus tissue lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27444-102 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317809于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 22 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 22 kDa. |
靶标
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功能
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. -
组织特异性
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). -
结构域
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
翻译后修饰
TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. -
细胞定位
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 12122 Mouse
- SwissProt: P70444 Mouse
- Unigene: 235081 Mouse
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别名
- Apoptic death agonist antibody
- Apoptotic death agonist BID antibody
- BH3 interacting domain death agonist antibody
see all
图片
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Western blot - Anti-Bid antibody [EPR27444-102] (ab317809)All lanes : Anti-Bid antibody [EPR27444-102] (ab317809) at 1/1000 dilution
Lane 1 : Untreated L-929 (mouse connective tissue fibroblast) whole cell lysate
Lane 2 : L-929 treated with 20ng/ml TNF alpha and 10µM BV-6 for 10h whole cell lysate
Lane 3 : C2C12 (mouse myoblast) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 6 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of Bid is downregulated in response to TNF alpha and BV-6 treatment (PMID: 11462023).
The identity of the higher MW bands at approximately 250 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-Bid antibody [EPR27444-102] (ab317809)](https://www.abcam.cn/ps/products/317/ab317809/Images/ab317809-542627-anti-bid-antibody-epr27444-102-western-blot-mouse-spleen-tissue-lysate-50-ug.jpg)
Western blot - Anti-Bid antibody [EPR27444-102] (ab317809)All lanes : Anti-Bid antibody [EPR27444-102] (ab317809) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse thymus tissue lysate
Lane 3 : Mouse lung tissue lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 6 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: mouse lung (PMID: 22446738).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Anti-Bid antibody [EPR27444-102] (ab317809)](https://www.abcam.cn/ps/products/317/ab317809/Images/ab317809-3-benefitsofrecombinantantibodies.jpg)
Anti-Bid antibody [EPR27444-102] (ab317809)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab317809 尚未被引用在任何文献中。