Anti-Bid抗体(ab272880)
Key features and details
- Rabbit polyclonal to Bid
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Bid抗体
参阅全部 Bid 一抗 -
描述
兔多克隆抗体to Bid -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Bid.
Database link: P55957 -
阳性对照
- WB: HEK-293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Rat2, RAW 264.7 and C2C12 whole cell lysate. IHC-P: U87 xenograft tissue. ICC/IF: A431 and HeLa cells. IP: Jurkat cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272880于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/3000. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/100 - 1/500. |
靶标
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功能
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. -
组织特异性
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). -
结构域
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
翻译后修饰
TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. -
细胞定位
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 637 Human
- Entrez Gene: 12122 Mouse
- Entrez Gene: 64625 Rat
- Omim: 601997 Human
- SwissProt: P55957 Human
- SwissProt: P70444 Mouse
- SwissProt: Q9JLT6 Rat
- Unigene: 591054 Human
see all -
别名
- Apoptic death agonist antibody
- Apoptotic death agonist BID antibody
- BH3 interacting domain death agonist antibody
see all
图片
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Western blot - Anti-Bid antibody (ab272880)All lanes : Anti-Bid antibody (ab272880) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 7 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.7.5 % SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody (ab272880)Paraffin-embedded U87 xenograft tissue stained for Bid using ab272880 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (ab272880)4% PFA-fixed A431 (human epidermoid carcinoma cell line) cells stained for Bid using ab272880 at 1/1000 dilution in ICC/IF.
Red: alpha Tubulin, a cytoskeleton marker.
Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-Bid antibody (ab272880)Immunoprecipitation of Bid protein from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts using 5 μg of ab272880 or ab137752.
Western blot analysis was performed using ab272880 diluted at 1:500, followed by anti-Rabbit IgG.Lane 1: Input.
Lane 2: Control IgG.
Lane 3: ab272880 IP.
Lane 4: ab137752 IP.
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Immunocytochemistry/ Immunofluorescence - Anti-Bid antibody (ab272880)2% paraformaldehyde/culture medium fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Bid using ab272880 at 1/1000 dilution in ICC/IF.
Blue: Hoechst 33343.
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Western blot - Anti-Bid antibody (ab272880)Anti-Bid antibody (ab272880) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg12% SDS-PAGE
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Western blot - Anti-Bid antibody (ab272880)All lanes : Anti-Bid antibody (ab272880) at 1/500 dilution
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.12% SDS-PAGE
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Western blot - Anti-Bid antibody (ab272880)All lanes : Anti-Bid antibody (ab272880) at 1/1000 dilution
Lane 1 : Non transfected HEK293T whole cell lysate
Lane 2 : Bid shRNA transfected HEK293T whole cell lysate
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG12% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab272880 被引用在 2 文献中.
- Xu XS et al. SCARF1 promotes M2 polarization of Kupffer cells via calcium-dependent PI3K-AKT-STAT3 signalling to improve liver transplantation. Cell Prolif 54:e13022 (2021). PubMed: 33686740
- Liang R et al. The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment. Cancers (Basel) 13:N/A (2021). PubMed: 34572804