Anti-Bid抗体(ab2388)
Key features and details
- Rabbit polyclonal to Bid
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Bid抗体
参阅全部 Bid 一抗 -
描述
兔多克隆抗体to Bid -
宿主
Rabbit -
特异性
This antibody detects full length (22 kDa) Bid. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, surrounding amino acid 37 of Human Bid. (Peptide available as ab38270.)
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常规说明
This product is manufactured by BioVision, an Abcam company and was previously called 3172 Bid Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa. |
靶标
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功能
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. -
组织特异性
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level). -
结构域
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
翻译后修饰
TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. -
细胞定位
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein. - Information by UniProt
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数据库链接
- Entrez Gene: 637 Human
- Omim: 601997 Human
- SwissProt: P55957 Human
- Unigene: 591054 Human
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别名
- Apoptic death agonist antibody
- Apoptotic death agonist BID antibody
- BH3 interacting domain death agonist antibody
see all
图片
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Western blot - Anti-Bid antibody (ab2388)Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours. Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab2388 被引用在 6 文献中.
- Hååg P et al. Caspase-2 is a mediator of apoptotic signaling in response to gemtuzumab ozogamicin in acute myeloid leukemia. Cell Death Discov 8:284 (2022). PubMed: 35690610
- Subhawa S et al. The Effects of Houttuynia cordata Thunb and Piper ribesioides Wall Extracts on Breast Carcinoma Cell Proliferation, Migration, Invasion and Apoptosis. Molecules 25:N/A (2020). PubMed: 32155880
- Liu CL et al. Reduced Nhe1 (Na+-H+ Exchanger-1) Function Protects ApoE-Deficient Mice From Ang II (Angiotensin II)-Induced Abdominal Aortic Aneurysms. Hypertension 76:87-100 (2020). PubMed: 32475310
- Yapasert R et al. Antitumor Efficacy of the Herbal Recipe Benja Amarit against Highly Invasive Cholangiocarcinoma by Inducing Apoptosis both In Vitro and In Vivo. Int J Mol Sci 21:N/A (2020). PubMed: 32784671
- Piao X et al. 1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway. Front Pharmacol 9:372 (2018). PubMed: 29725297
- Shinozaki-Ushiku A et al. Profiling of Virus-Encoded MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma and Their Roles in Gastric Carcinogenesis. J Virol 89:5581-91 (2015). PubMed: 25740983