AB287162

重组Anti-BFL-1/GRS抗体[EPR24587-176] - BSA and Azide free

Anti-BFL-1/GRS antibody [EPR24587-176] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal BFL-1/GRS antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

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BCL2L5, BFL1, GRS, HBPA1, BCL2A1, Bcl-2-related protein A1, Bcl-2-like protein 5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS, Bcl2-L-5

2 Images

Lab

Immunoprecipitation - Anti-BFL-1/GRS antibody [EPR24587-176] - BSA and Azide free (AB287162)

This data was developed using ab287160, the same antibody clone in a different buffer formulation.

BFL-1/GRS was immunoprecipitated from 0.35 mg SK-MEL-28 (human malignant melanoma) whole cell lysate 10 μg with ab278160 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278160 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SK-MEL-28 (human malignant melanoma) whole cell lysate 10 μg

Lane 2 : ab278160 IP in SK-MEL-28 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab278160 in SK-MEL-28 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 50 seconds

All lanes:

Immunoprecipitation - Anti-BFL-1/GRS antibody [EPR24587-176] (<a href='/products/primary-antibodies/bfl-1-grs-antibody-epr24587-176-ab287160'>ab287160</a>)

Predicted band size: 20 kDa

Observed band size: 18 kDa

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Lab

Western blot - Anti-BFL-1/GRS antibody [EPR24587-176] - BSA and Azide free (AB287162)

This data was developed using ab287160, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HCT116, HL-60, MOLT-4, PC-3 (PMID : 23447565 )

Exposure time : 37 seconds

All lanes:

Western blot - Anti-BFL-1/GRS antibody [EPR24587-176] (<a href='/products/primary-antibodies/bfl-1-grs-antibody-epr24587-176-ab287160'>ab287160</a>) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma monocyte) whole cell lysate at 60 µg

Lane 2:

SK-MEL-28 (human malignant melanoma) whole cell lysate at 60 µg

Lane 3:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 60 µg

Lane 4:

HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate at 60 µg

Lane 5:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 60 µg

Lane 6:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

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不同偶联物与剂型 (1)

Unconjugated

Anti-BFL-1/GRS antibody [EPR24587-176]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24587-176

不含载体蛋白

Yes

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab287162 is the carrier free version of ab287160

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity). Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (By similarity).

See full target information BCL2A1

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-BFL-1/GRS抗体[EPR24587-176] - BSA and Azide free