AB287160

重组Anti-BFL-1/GRS抗体[EPR24587-176]

Anti-BFL-1/GRS antibody [EPR24587-176]

20ul selling size
RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal BFL-1/GRS antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

查看别名

BCL2L5, BFL1, GRS, HBPA1, BCL2A1, Bcl-2-related protein A1, Bcl-2-like protein 5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS, Bcl2-L-5

2 Images

Lab

Immunoprecipitation - Anti-BFL-1/GRS antibody [EPR24587-176] (AB287160)

BFL-1/GRS was immunoprecipitated from 0.35 mg SK-MEL-28 (human malignant melanoma) whole cell lysate 10 ug with ab278160 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278160 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SK-MEL-28 (human malignant melanoma) whole cell lysate 10 μg

Lane 2 : ab278160 IP in SK-MEL-28 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab278160 in SK-MEL-28 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 50 seconds

All lanes:

Immunoprecipitation - Anti-BFL-1/GRS antibody [EPR24587-176] (ab287160)

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

Lab

Western blot - Anti-BFL-1/GRS antibody [EPR24587-176] (AB287160)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : HCT116, HL-60, MOLT-4, PC-3 (PMID : 23447565)

Exposure time : 37 seconds

All lanes:

Western blot - Anti-BFL-1/GRS antibody [EPR24587-176] (ab287160) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma monocyte) whole cell lysate at 60 µg

Lane 2:

SK-MEL-28 (human malignant melanoma) whole cell lysate at 60 µg

Lane 3:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 60 µg

Lane 4:

HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate at 60 µg

Lane 5:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 60 µg

Lane 6:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 18 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-BFL-1/GRS antibody [EPR24587-176] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24587-176

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection (By similarity). Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (By similarity).

See full target information BCL2A1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 19:20221007 PubMed39711976

2024

ZAG promotes colorectal cancer cell proliferation and epithelial-mesenchymal transition by promoting lipid synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Maotao Xu,Xingzheng Jin,Zhouli Shen

Dose-response : a publication of International Hormesis Society 21:15593258231197101 PubMed37654726

2023

Scutellarin Inhibits Glioblastoma Growth in a Dose-dependent Manner by Suppressing the p63 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Chen,Wenlan Li

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-BFL-1/GRS抗体[EPR24587-176]