Anti-beta Tubulin抗体[1E1-E8-H4] - Microtubule Marker (ab131205)
![Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)](https://www.abcam.cn/ps/products/131/ab131205/Images/ab131205-316810-anti-beta-tubulin-antibody-1e1-e8-h4-western-blot.jpg "Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)")
Key features and details
- Mouse monoclonal [1E1-E8-H4] to beta Tubulin - Microtubule Marker
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-beta Tubulin抗体[1E1-E8-H4] - Microtubule Marker
参阅全部 beta Tubulin 一抗 -
描述
小鼠单克隆抗体[1E1-E8-H4] to beta Tubulin - Microtubule Marker -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, African green monkey -
免疫原
Recombinant fragment corresponding to Human beta Tubulin.
Database link: P07437 -
阳性对照
- A431, HeLa and Jurkat cell lysates.
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常规说明
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading... -
纯度
Concentrated Culture Supernatant -
克隆
单克隆 -
克隆编号
1E1-E8-H4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131205于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) |
1/300.
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| WB | (3) |
1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
|
| 说明 |
|---|
|
ICC/IF
1/300. |
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WB
1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
组织特异性
Ubiquitously expressed with highest levels in spleen, thymus and immature brain. -
疾病相关
Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1 -
序列相似性
Belongs to the tubulin family. -
结构域
The highly acidic C-terminal region may bind cations such as calcium. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. -
细胞定位
Cytoplasm, cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 203068 Human
- Entrez Gene: 22154 Mouse
- Entrez Gene: 29214 Rat
- Omim: 191130 Human
- SwissProt: P07437 Human
- SwissProt: P99024 Mouse
- SwissProt: P69897 Rat
- Unigene: 636480 Human
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别名
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
see all
图片
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Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/5000 dilution
Lane 1 : C6 Cell lysate
Lane 2 : K562 Cell lysate
Lane 3 : COS7 Cell lysate
Lane 4 : 3T3 Cell lysate
Lane 5 : HeLa Cell lysate
Predicted band size: 49 kDa -
![Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)](https://www.abcam.cn/ps/products/131/ab131205/Images/ab131205-233440-anti-beta-tubulin-antibody-1e1-e8-h4-western-blot.jpg)
Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/500 dilution
Lane 1 : COS7 cell lysate
Lane 2 : LNCap cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : C2C12 cell lysate
Predicted band size: 49 kDaThis image was produced with antibody produced in ascites.
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![Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)](https://www.abcam.cn/ps/products/131/ab131205/Images/ab131205-145499-anti-beta-tubulin-antibody-1e1-e8-h4-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)Immunocytochemistry stain of Hela using ab131205(1:300). This image was produced with antibody produced in ascites.
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![Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)](https://www.abcam.cn/ps/products/131/ab131205/Images/ab131205-145498-anti-beta-tubulin-antibody-1e1-e8-h4-western-blot.jpg)
Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)ab131205 stability test under 37ºC for 50 hours compared to 4°C for 50 hours. This image was produced with antibody produced in ascites.
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![Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)](https://www.abcam.cn/ps/products/131/ab131205/Images/ab131205-145500-anti-beta-tubulin-antibody-1e1-e8-h4-western-blot.jpg)
Western blot - Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205)All lanes : Anti-beta Tubulin antibody [1E1-E8-H4] - Microtubule Marker (ab131205) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Predicted band size: 49 kDa
Observed band size: 49 kDaThis image was produced with antibody produced in ascites.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab131205 被引用在 20 文献中.
- Liu Y et al. Upregulation of SLITRK5 in patients with epilepsy and in a rat model. Synapse 77:e22266 (2023). PubMed: 36811190
- Huang P et al. YTHDF1 Attenuates TBI-Induced Brain-Gut Axis Dysfunction in Mice. Int J Mol Sci 24:N/A (2023). PubMed: 36835655
- Ward EN et al. Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging. Nat Commun 13:7836 (2022). PubMed: 36543776
- Caron NS et al. Cerebrospinal fluid mutant huntingtin is a biomarker for huntingtin lowering in the striatum of Huntington disease mice. Neurobiol Dis 166:105652 (2022). PubMed: 35143966
- Dagda RK et al. Intranasal Administration of Forskolin and Noopept Reverses Parkinsonian Pathology in PINK1 Knockout Rats. Int J Mol Sci 24:N/A (2022). PubMed: 36614135